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US 2026/0298925 A1Published application
Systems, methods, and compositions for the detection of microbes
AssigneeSD Systems, Inc.
InventorsElijah SHARPE, Darrin CLAWSON, Ashley REMIGIO, Mitchell LOW, Jenny KELLY
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Abstract
Provided are non-naturally occurring systems, methods, and compositions for the detection of microbes. The disclosure relates to the detection of an antigen specific to a microbe, such as a foodborne, an environment-borne, and a bloodborne bacteria, using capture antibody and moiety, detector antibody and moiety, and a light-emitting particle.
Claims39
A method of detecting an antigen, the method comprising:
- A:
- mixing a solution comprising a capture antibody, a detector antibody, and a sample suspected of containing the antigen, wherein:
- the capture antibody is linked to a capture moiety by a functional group and specifically binds to the antigen in conditions sufficient to form a first complex between the antigen and the capture antibody;
- and
- the detector antibody is linked to a detection moiety and specifically binds to the antigen in conditions sufficient to form a second complex between the first complex and the detector antibody, wherein the detection moiety is a light-emitting microsphere;
- separating unbound detector antibody linked to the detection moiety from the second complex;
- and
- detecting the light-emitting microspheres on the second complex by spectroscopy, thereby detecting the antigen;
- B:
- mixing a solution comprising a capture antibody, a detector antibody, and a sample suspected of containing the antigen, wherein the capture antibody is linked to a capture moiety by a functional group, and the detector antibody is linked to a detection moiety, wherein the detection moiety is a light-emitting microsphere;
- separating the capture antibody from both the detector antibody and sample;
- performing a baseline analysis by detecting the light-emitting microspheres from the detector antibody by spectroscopy;
- mixing and incubating the capture antibody, the detector antibody, and the sample, wherein the capture antibody specifically binds to the antigen in conditions sufficient to form a first complex between the antigen and the capture antibody, and wherein the detector antibody specifically binds to the antigen in conditions sufficient to form a second complex between the first complex and the detector antibody;
- separating unbound detector antibody from the second complex;
- performing a final analysis by detecting the light-emitting microspheres on the second complex and/or the light-emitting microspheres from the unbound detector antibody by spectroscopy;
- and
- comparing the baseline analysis and the final analysis,
- thereby detecting the antigen;
- or
- C:
- mixing a solution comprising a capture antibody, a detector antibody, and a sample suspected of containing the antigen, wherein the capture antibody is linked to a capture moiety by a functional group, and the detector antibody is linked to a detection moiety, wherein the detection moiety is a light-emitting microsphere;
- performing a baseline analysis by detecting the light-emitting microspheres from the detector antibody of the mixture by spectroscopy;
- incubating the capture antibody, the detector antibody, and the sample, wherein the capture antibody specifically binds to the antigen in conditions sufficient to form a first complex between the antigen and the capture antibody, and wherein the detector antibody specifically binds to the antigen in conditions sufficient to form a second complex between the first complex and the detector antibody;
- separating unbound detector antibody from the second complex;
- performing a final analysis by detecting the light-emitting microspheres on the second complex and/or the light-emitting microspheres from the unbound detector antibody by spectroscopy;
- and
- comparing the baseline analysis and the final analysis,
- thereby detecting the antigen.
25 more elements
An apparatus for the detection of an antigen, comprising:
- a solution comprising a capture antibody, a detector antibody, and a sample suspected of containing the antigen, wherein:
- the capture antibody is linked to a capture moiety by a functional group and specifically binds to the antigen in conditions sufficient to form a first complex between the antigen and the capture antibody;
- and
- the detector antibody is linked to a detection moiety and specifically binds to the antigen in conditions sufficient to form a second complex between the first complex and the detector antibody, wherein the detection moiety is a light-emitting microsphere;
- a plurality of sample containers, wherein each sample container is configured for:
- receiving the solution comprising the capture antibody, the detector antibody, and the sample suspected of containing the antigen;
- receiving illumination at a first wavelength range from a light source;
- and
- transmitting radiation from the detection moiety;
- and
- an optical instrument comprising:
- a plurality of sample container receiving positions, each for receiving a respective one of the plurality of sample containers;
- the light source adjacent to at least one of the sample containers receiving positions for providing illumination at the first wavelength range and in a first illumination direction through a respective sample container once disposed within the respective sample container receiving position for causing radiation from the detection moiety bound within the detection platform;
- a carousel with one or more repositionable mirrors for focusing the illumination from the light source;
- and
- a photodetector disposed adjacent to the sample container receiving positions for selectively detecting the energy of light radiated from the detection moiety within the respective sample container in response to illumination from the light source and for generating an electrical signal corresponding to the detected energy of the radiated light in response thereto.
13 more elements
An apparatus for geolocating a source of potential foodborne, environment-borne, and/or bloodborne bacteria, comprising:
- a solution comprising a capture antibody, a detector antibody, and a sample suspected of containing the bacteria, wherein:
- an antigen is located on the surface of the bacteria;
- the capture antibody is linked to a capture moiety by a functional group and specifically binds to the antigen in conditions sufficient to form a first complex between the antigen and the capture antibody;
- and
- the detector antibody is linked to a detection moiety and specifically binds to the antigen in conditions sufficient to form a second complex between the first complex and the detector antibody, wherein the detection moiety is a light-emitting microsphere;
- one or more sample containers;
- an optical instrument;
- and
- a global positioning system (GPS).
6 more elements
Dependencies are inferred from the claim text.