Rapid glycan sample preparation methods, system and kits
OwnerMohammadali Masigol, Mastooreh Chamanian +2inventors
InventorsMohammadali Masigol, Mastooreh Chamanian, Jianli Zhao, Mihir Mahendrabhai Thakar
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Abstract
Disclosed are methods, systems and kits for the separation and recovery of glycans from a glycosylated substrate. Magnetic particles are incorporated in a sample containing the glycosylated substrate. The magnetic particles are utilized to efficiently mix the sample through various steps of the separation and recovery workflow. The magnetic particles are surface 5 functionalized so as to attach released glycans on the surface of the particles. Disclosed systems include a device that is capable of generating an electromagnetic field, wherein the samples containing the magnetic particles are placed for processing.
Claims20
A method for recovery of glycans from a glycosylated substrate, the method comprising:
- a) adding an enzymatic agent to a sample containing a glycosylated substrate and releasing glycans from the glycosylated substrate;
- b) labelling released glycans;
- c) attaching labelled glycans to magnetic particles;
- d) disassociating the labelled glycans from the magnetic particles;
- and
- wherein a magnetic field is applied during each of the steps a) through d).
A system for recovery of glycans from a glycosylated substrate, the system comprising:
- a device capable of generating a magnetic field;
- a plurality of magnetic particles;
- at least one enzymatic agent;
- and
- at least one labelling reagent.
Dependencies are inferred from the claim text.