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US 2026/0298910 A1Published application
Immunogenicity screening method
OwnerSathy BALU-IYER, Nicole JARVIinventors
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Abstract
Provided is a method for determining if a protein will produce an immunogenic response. The immunogenic response may be correlated by the migration of dendritic cells in the presence of the protein. The method may be used as a screening tool to assess immunogenicity risk for proteins that could be administered subcutaneously.
Claims22
A method for determining dendritic cell migration comprising:
- contacting a known amount of dendritic cells with a therapeutic protein at a first protein concentration in a first chamber, wherein the first chamber is connected to and adjacent to a second chamber and the first chamber and second chamber are separated by a porous membrane, wherein the second chamber contains the therapeutic protein at a second protein concentration and one or more chemokines and does not contain any dendritic cells;
- incubating the first chamber and second chamber;
- isolating migrated dendritic cells from the second chamber;
- staining the migrated dendritic cells from the second chamber;
- and
- counting the migrated dendritic cells from the second chamber,
- wherein the counted cells have migrated from the first chamber.
4 more elements
Dependencies are inferred from the claim text.