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US 2026/0298893 A1Published application
Fed-batch ivt assay for ntp and rna via hplc
AssigneeAldevron, LLC
InventorsMustafa Fakhri, Rebecca Young, Ryan Lagacy, Jonathan William Cooper, Aakanksha Singh Parihar
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Abstract
Methods for monitoring an in vitro transcription (IVT) process include obtaining a sample from an IVT process at a first timepoint. The sample is run on a liquid chromatography (LC) column, the LC column characterized by being a silica column with C18 bonding and a Lewis acid/Lewis base endcapping. One or more peaks are quantitated against a first calibration curve.
Claims20
A method of monitoring an in vitro transcription (IVT) process, the method comprising:
- obtaining a sample from an IVT process at a first timepoint;
- running the sample on a liquid chromatography (LC) column, the LC column characterized by being a silica column with C18 bonding and a Lewis acid/Lewis base endcapping;
- and
- quantitating one or more peaks against a first calibration curve.
1 more element
A method of in vitro transcription, the method comprising:
- generating a DNA template;
- initiating a transcription reaction including one or more reagents;
- monitoring the transcription reaction by:
- obtaining a sample from an IVT process at a first timepoint;
- running the sample on a liquid chromatography (LC) column, the LC column characterized by being a silica column with C18 bonding and a Lewis acid/Lewis base endcapping;
- quantifying one or more peaks against a calibration curve;
- and
- purifying product RNA.
5 more elements
Dependencies are inferred from the claim text.