Kit for early detection of colorectal cancer dna using lateral flow assay

A kit for detecting colorectal cancer DNA using a lateral flow assay, comprising:

• a loading pad on which a blood sample is loaded;
• a multilayer filter located at the bottom of the loading pad to remove impurities such as red blood cells from the blood sample and pass only serum;
• a loop-mediated isothermal amplification (LAMP) device located at the bottom of the multilayer filter to amplify a specific target circulating tumor DNA (ctDNA) fragment in colorectal cancer mutant DNA using single nucleotide polymorphism (SNP) typing;
• a nitrocellulose membrane located at the bottom of the LAMP device and through which the amplified SNP sequence moves;
• a first probe of gold nanoparticles that binds to the SNP sequence moving along the nitrocellulose membrane to form a DNA-AuNP complex;
• and
• a second probe that indicates whether to be color-developed to operate normally by allowing the extra blood sample to move to a control line, so as to detect whether colorectal cancer DNA exists in the blood sample based on the color-development of the first and second probes.