Method for detecting a substance of interest using nucleic acid aptamer beacons
AssigneeHonda Motor Co., Ltd.
InventorsTatsuo NAKAMURA, Yuko NAKAMURA, Tadahisa NAKAMURA, Tsuyumi NISHIYAMA, Toshiaki SUMI
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Abstract
A method to the sensitivity of nucleic acid aptamer beacons, which in one respect involves detecting a target substance using a nucleic acid aptamer beacon. The wherein the method includes: (A) a step of mixing a target substance, a nucleic acid aptamer beacon, and a response-enhancing nucleic acid; and (B) a step of measuring light emission from the mixture, wherein the response-enhancing nucleic acid includes a region hybridizable to a portion of at least one of the region of the first nucleic acid and the region of the second nucleic acid of the nucleic acid aptamer.
Claims12
A method for detecting a target substance using a nucleic acid aptamer beacon,
- the nucleic acid aptamer beacon comprising:
- (1) a region of a first substance that emits fluorescence,
- (2) a region of a second substance that modulates light emission by the first substance,
- (3) a region of a first nucleic acid linked directly or indirectly to the first substance,
- (4) a region of a second nucleic acid linked directly or indirectly to the second substance, and
- (5) a target substance-binding region configured to bind to the target substance and cause a conformational change in the nucleic acid aptamer beacon,
- wherein the region of the first nucleic acid and the region of the second nucleic acid may be integrated or may be present separately,
- the region of the first nucleic acid and the region of the second nucleic acid each comprise at least one region of a partial sequence hybridizable to each other,
- wherein the method comprises:
- (A) a step of mixing the target substance, the nucleic acid aptamer beacon, and a response-enhancing nucleic acid;
- and
- (B) a step of measuring light emission from the mixture,
- wherein the response-enhancing nucleic acid comprises a region hybridizable to a portion of at least one of the region of the first nucleic acid and the region of the second nucleic acid.
Dependencies are inferred from the claim text.