Recombinant enzyme for the accurate insertion of dna sequences in eukaryotic cells

A method of editing genomic material in a target organism, comprising:

• providing a recombinant enzyme, the enzyme including:
• an HIV integrase (HIV IN) tetrameric structure;
• and
• two or more Transcription Activator Like Effectors (TALEs) bound to the HIV IN tetramer,
• binding one or more polynucleotides to the recombinant enzyme to form a gene editing construct;
• administering an effective amount of the gene editing construct to the target organism;
• transporting a concentration of the gene editing construct to a cellular nucleus in the target organism;
• localizing the gene editing construct on a target DNA structure via the TALEs;
• and
• inserting the one or more polynucleotides into the genomic material of the target organism at the target DNA structure.

A recombinant enzymatic gene editing system, comprising:

• a recombinant enzyme, the enzyme including:
• an HIV integrase (HIV IN) tetramer including a first inner monomer and a second inner monomer and a first outer monomer and a second outer monomer, wherein the first inner monomer and the second inner monomer are bound to each other and the first outer monomer is bound to the first inner monomer and the second outer monomer is bound to the second inner monomer;
• and
• a first Transcription Activator Like Effector (TALEs bound to an N-terminal region of the first outer monomer via a first polypeptide linker and a second TALE bound to an N-terminal region of the second outer monomer via a second polypeptide linker,
• one or more polynucleotides bound to at least one of the first inner monomer and the second inner monomer to form a gene editing construct, and
• a lentiviral capsid encapsulating the gene editing construct.