ARTIFICIAL SOLEMOVIRIDAE SATELLITE RNAs

A recombinant RNA molecule comprising from 5′ terminus to 3′ terminus:

• (a) a 5′ RNA replication element recognized by a Solemoviridae RNA-dependent RNA polymerase (RdRP);
• (b) a cargo RNA molecule;
• and
• (c) a 3′ RNA replication element recognized by the RdRP;
• wherein the 5′ RNA replication element, the cargo RNA molecule, and the 3′ RNA replication element are operably linked;
• wherein the cargo RNA molecule is heterologous to the 5′ RNA replication element and the 3′ RNA replication element;
• and, optionally, wherein:
• (i) the 5′ RNA replication element and the 3′ RNA replication element are obtained from the same Solemoviridae genome or from Solemoviridae genomes having at least 85%, 90%, 95%, 98%, or 99% sequence identity to one another;
• (ii) the 5′ RNA replication element, the 3′ RNA replication element, and the RdRP are obtained from the same Solemoviridae genome or from Solemoviridae genomes having at least 85%, 90%, 95%, 98%, or 99% sequence identity to one another;
• or (iii) the 5′ RNA replication element, the 3′ RNA replication element, and/or the RdRP coding region are obtained from different Solemoviridae genomes, and wherein the members of each respective set of the 5′ RNA replication elements, 3′ RNA replication elements, and/or the RdRP coding regions have at least 85%, 90%, 95%, 98%, or 99% sequence identity to one another.