US 2026/0297553 A1 · Improved Enzyme Sensor Membrane for Lactose

Abstract

A trilaminar membrane invention to quantitate lactose in conjunction with a potentiostatic amperometric cell. The invention features a support layer, an immobilized enzyme layer, and an electrode-facing exclusion layer. The support layer is a homogeneous, semi-permeable polycarbonate film that controls diffusion rates of analyte into the enzyme reaction layer. The analyte is then oxidatively processed to generate hydrogen peroxide (H2O2) by the immobilized 2-enzyme system comprised of β-galactosidase and glucose oxidase. Hydrogen peroxide then diffuses through the permselective exclusion layer to react at a poised sense electrode probe to generate an electrical signal for analyte quantitation. The thin sensing membrane is affixed to an O-ring for mounting on the face of the electrode probe.

Claims15

Dependent claims

A laminated membrane to quantitate lactose in an analyte sample using a biochemistry analyzer, comprising:

a support layer configured to control a diffusion rate of an analyte sample having lactose into the laminated membrane;
an exclusion layer configured to diffuse hydrogen peroxide (H2O2) from the laminated membrane;
and
an enzyme reaction layer having a 2-enzyme system configured between the support layer and the exclusion layer, the 2-enzyme system having β-galactosidase and glucose oxidase configured to process oxidatively the analyte sample having the lactose and produce the hydrogen peroxide (H2O2) for diffusing through the exclusion layer and from the laminated membrane and reacting with a sensor electrode to quantitate the lactose in the analyte sample with the biochemistry analyzer.

1 more element

Dependencies are inferred from the claim text.

Improved Enzyme Sensor Membrane for Lactose

Life of this patent

Improved Enzyme Sensor Membrane for Lactose

Life of this patent

Abstract

Claims15