3d epithelial organoid culture systems and methods
AssigneeThe Regents of the University of California
InventorsHing Jii MEA, Jiandi WAN
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Abstract
Disclosed herein are 3D culture systems and methods for producing epithelial tubular organoids, such as intestinal organoids. Also disclosed is a screening method that involves contacting elongated epithelial tubes produced from the disclosed systems and methods with a candidate agent, culturing the elongated epithelial tube in a growth medium under growth conditions, and evaluating the elongated epithelial tube for a physiological effect.
Claims20
A 3D organoid culture system comprising a filament having a bioactive hydrogel core covered by an inert hydrogel sheath, wherein the bioactive hydrogel core is an extracellular matrix hydrogel, and wherein the inert hydrogel has a diffusion rate for growth factors of from 1×10−7 to 9×10−7 cm2/s.
Dependencies are inferred from the claim text.