Continuous oligonucleotide synthesizer and method for synthesizing oligonucleotide using the same

A continuous oligonucleotide synthesizer, comprising:

• a raw material container that holds a mixture of at least two types of protected nucleoside blocks used as a raw material;
• a first reagent container that holds a first reagent for performing nucleotide chain elongation by mutually reacting the at least two types of protected nucleoside blocks;
• a second reagent container that holds a second reagent for an oxidizing or sulfurizing reaction of a phosphite binding moiety of an elongated nucleotide resulting from the chain elongation;
• a third reagent container that holds a third reagent for a deprotecting reaction of the elongated nucleotide having been oxidized or sulfurized;
• a first joint that connects a pipe extending from the raw material container to a pipe extending from the first reagent container;
• a first reaction tube that connects the first joint to a second joint connected to a pipe extending from the second reagent container;
• and
• a second reaction tube that connects the second joint to a third joint connected to a pipe extending from the third reagent container,
• wherein each of the raw material, the first reagent, the second reagent, and the third reagent is in the form of a solution,
• liquid feeding devices are connected the pipes extending from the raw material container, the first reagent container, the second reagent container, and the third reagent container, respectively, wherein the liquid feeding device feeds the solution held in the container to the reaction tube connected to the corresponding pipe,
• a raw material mixture obtained by combining, in the first joint, the solution of the raw material and the solution of the first reagent is introduced to the first reaction tube,
• a nucleotide chain elongation reaction is performed using the raw material mixture in the first reaction tube to introduce a solution containing a resultant chain elongated product to the second reaction tube,
• the oxidizing or sulfurizing reaction of the chain elongated product is performed in the second reaction tube, and
• oligonucleotide synthesis is continuously performed by continuously supplying the raw material, the first reagent, and the second reagent.