In line cleaning in place process for alternating tangential flow filter

Abstract

The present invention provides a process for an in line cleaning in place (CIP) of an alternating tangential flow (ATF) filter in a filtration module, the filtration module being in communication with a bioreactor. The process comprises: flowing a dilute NaOH through the ATF filter, the dilute NaOH being 0.1 to 0.5 N NaOH, the volume of NaOH being three to five times the hold up volume of the filter and the flow rate of NaOH being 80-200 ml/L; flushing the filter with a sterile water, the volume of water being flushed being ten to twenty times the hold up volume of the filter; and equilibrating the filter with a media. The present invention also provides a system for an in line CIP of an alternating tangential flow (ATF) filter in a filtration module, the filtration module being in communication with a bioreactor.

Dependencies are inferred from the claim text.