Golden lipid nanoparticles for gene therapy
AssigneeUniversidaddelpaíSvasco/Euskalherrikounibertsitatea
InventorsAna DEL POZO RODRÍGUEZ, Alicia RODRÍGUEZGASCÓN, Itziar GÓMEZAGUADO, Mónica VICENTEPASCUAL, María Angeles SOLINÍSASPIAZU
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Abstract
The invention provides solid lipid nanoparticles (SLNs) which comprise gold, and are useful as vectors for the transfection of different types of nucleic acids. Different methods for obtaining such SLNs are also disclosed.
Claims20
A process for the preparation of a solid lipid nanoparticle (SLN) comprising:
- a) preparing a suspension of precursor SLN, said precursor SLN comprising:
- a lipid solid at room temperature at the core of the precursor SLN;
- a cationic surfactant;
- and
- a non-ionic surfactant;
- b) preparing a solution comprising:
- a nucleic acid;
- gold;
- and
- if required in the SLN to be obtained, a peptide with a net positive charge and/or a polysaccharide;
- c) mixing the suspension of precursor SLN obtained in step (a) with the solution obtained in step (b),
- wherein the SLN comprises a lipid solid at room temperature at the core of the SLN, at least one cationic surfactant, at least one non-ionic surfactant, gold, and a nucleic acid.
A process for the preparation of a solid lipid nanoparticle (SLN) comprising:
- a) preparing a suspension of precursor gold SLN, said precursor gold SLN comprising:
- a lipid solid at room temperature at the core of the precursor gold SLN;
- a cationic surfactant;
- a non-ionic surfactant;
- and
- gold;
- b) preparing a solution comprising:
- a nucleic acid;
- and
- if required in the SLN to be obtained, a peptide with a net positive charge and/or a polysaccharide;
- c) mixing the suspension of precursor gold SLN obtained in step (a) with the solution obtained in step (b),
- wherein the SLN comprises a lipid solid at room temperature at the core of the SLN, at least one cationic surfactant, at least one non-ionic surfactant, gold, and a nucleic acid.
A method of gene therapy, said method comprising administering a therapeutically effective amount of a solid lipid nanoparticle (SLN) to a patient in need of such therapy, wherein the SLN comprises a lipid solid at room temperature at the core of the SLN, at least one cationic surfactant, at least one non-ionic surfactant, gold, and a nucleic acid.
(canceled)
Dependencies are inferred from the claim text.