Chimeric polypeptides, extracellular vesicles comprising the same, and uses thereof

A chimeric polypeptide comprising:

• i) an amino acid sequence of a protein of interest, wherein said protein of interest is selected from a type II transmembrane protein, a protein from the TNF or TNFR superfamily, a protein from the TGF-β superfamily, a protein from the neurotrophin family, and a protein from the CTRP family selected from the group consisting of:
• CTRP1, CTRP2, CTRP3, CTRP4, CTRP5, CTRP6, CTRP7, CTRP8, CTRP9, CTRP10, CTRP11, CTRP12, CTRP13, CTRP14 and CTRP15;
• ii) optionally an amino acid sequence of a transmembrane domain of a transmembrane protein;
• and
• iii) an amino acid sequence of a pilot peptide interacting with the Endosomal Sorting Complexes Required for Transport (ESCRT) cellular machinery and/or an amino acid sequence of a sub-membrane targeting domain,
• wherein the components i), ii) and iii) are organized in the chimeric polypeptide from C-terminal to N-terminal.