Il-12 mutants with reduced toxicity, compositions thereof and methods of using the same
OwnerJunhao YANG, Amandeep Kaur GAKHAL +3inventors
InventorsJunhao YANG, Amandeep Kaur GAKHAL, Alice YAM, Daniel CALARESE, Robert HENNINGSEN
Life of this patent
Estimating the term…
Abstract
The present disclosure provided to IL-12 polypeptides with at least one amino acid substitution, pharmaceutical compositions, diagnostic compositions, and kits containing the polypeptides, nucleic acids and expression vectors encoding the polypeptides, cells comprising the same, and methods of using the polypeptides, nucleic acids, expression vectors, and cells for therapeutic and diagnostic purposes.
Claims39
A variant IL-12 p40 subunit polypeptide, wherein the variant IL-12 p40 subunit polypeptide comprises at least one amino acid substitution relative to SEQ ID NO:
- 1, wherein the at least one amino acid substitution is selected from the group consisting of:
- W16X, Y17A, P21A, D42A, D42K, Q43A, S44A, S45A, E46A, E46K, K59A, K59E, K85E, E87A, E87K, D88A, D88K, 190A, W91A, D94X, K103A, K103E, N104A, K105A, K105E, D162A, D162K, H195A, H195E, K196A, K196E, L197A, K198E, N201A, T203A, C253X, K259Y, K265Y, I278Y, and combinations thereof, wherein X indicates any amino acid other than the SEQ ID NO:
- 1 amino acid at the indicated position.
Dependencies are inferred from the claim text.