US 2026/0294998 A1Published application

Plant-based amyloid fibrils, their Preparation Method, Composite Film, and Composite Coating Preservative

AssigneeZhejiang University

InventorsWenjun WANG, Tiange PAN, Hao WANG, Congyi XU, Haiqi CHEN, Kai FU, Lifen ZHANG, Ruiling LV, Songfeng YU, Jianwei ZHOU, Donghong LIU

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Abstract

A method for preparing plant-based amyloid fibrils includes mixing a plant protein isolate with water, adjusting the pH to 1.5-2.5, and then performing solid-liquid separation. The resulting liquid material containing the plant protein isolate is subjected to a fibrillization treatment to obtain the plant-based amyloid fibrils. According to the growth kinetics curve, the fibrillization treatment sequentially includes a first incubation treatment, an ultrasonic treatment, and a second incubation treatment. The duration of the first incubation ranges from 0 h to the end of the exponential phase, and both the first and second incubation treatments are carried out under non-ultrasonic conditions. When the duration of the first incubation treatment is 0 h, the power density of the ultrasonic treatment is ≥2.5 W/mL. The method enables simultaneous regulation of the fibril growth process and morphology, and reduces the proportion of rigid fibrils.

Claims8

Dependent claims

A method for preparing plant-based amyloid fibrils, comprising:

1) mixing a plant protein isolate with water to obtain an aqueous solution of the plant protein isolate, wherein the plant protein isolate comprises pea protein isolate;
2) adjusting the pH of the aqueous solution of the plant protein isolate to 1.5-2.5, followed by solid-liquid separation to obtain a liquid material containing the plant protein isolate;
and
3) subjecting the liquid material containing the plant protein isolate to a fibrillization treatment to obtain the plant-based amyloid fibrils;
wherein the fibrillization treatment sequentially comprises a first incubation treatment, an ultrasonic treatment, and a second incubation treatment;
and both the first and second incubation treatments are performed under non-ultrasonic conditions;
wherein temperatures of the first and second incubation treatments are independently in a range of 75-85° C.;
a total duration of the first and second incubation treatments is in a range of 45-50 h;
and the first and second incubation treatments are performed under stirring;
wherein a temperature of the ultrasonic treatment is in a range of 0-4° C.;
a total duration of the ultrasonic treatment is in a range of 1-3 min;
the ultrasonic treatment is performed with an interval of 1-3 seconds after every 1-3 seconds of ultrasonic irradiation;
and
wherein the fibrillization treatment comprises any one of the following:
1) a duration of the first incubation treatment is 0 h, and an ultrasonic power density of the ultrasonic treatment is in a range of 2.6-4 W/mL;
2) the duration of the first incubation treatment is in a range of 4-5 h, and the ultrasonic power density of the ultrasonic treatment is in a range of 3-4 W/mL;
and
3) the duration of the first incubation treatment is in a range of 25-26.5 h, and the ultrasonic power density of the ultrasonic treatment is in a range of 2-2.2 W/mL.

15 more elements

Dependencies are inferred from the claim text.