Micronized compositions for wound healing prepared from human umbilical cord tissue and intact amnion-chorion tissue having an intact intermediate spongy layer positioned there between

A sterile micronized composition prepared from intact human placental tissue, the sterile micronized composition comprising:

• (a) micronized human umbilical cord;
• (b) micronized human amnion;
• (c) micronized human chorion;
• and
• (d) micronized human intermediate spongy layer;
• wherein the composition is prepared from intact placental tissue comprising a human umbilical cord and, in cross-section, a human amnion layer, human chorion layer, and an intact human intermediate spongy layer positioned between and connecting the human amnion layer to the human chorion layer such that immediately before micronization the human amnion layer and the human chorion layer are not separated from one another;
• wherein the composition is prepared without the use of exogenous enzymes;
• and
• wherein the composition comprises at least three of the following:
• (a) IL-1ra ranging from 45 to 7,800 pg/mL;
• (b) HGF ranging from 300 to 14,500 pg/mL;
• (c) HA ranging from 2.0×103 to 9.3×108 pg/mL;
• (d) GAGs;
• and
• (e) Collagen.