Methods for stem cell transplantation
AssigneeThe UAB Research Foundation
InventorsLawrence S. Lamb, Shin MINEISHI, Ayman SAAD
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Abstract
The present disclosure provides methods of hematopoietic stem cell transplantation (HSCT). In particular, the present disclosure provides a method of HSCT using a combination of an in-vivo T-cell depletion method, with an ex-vivo method of γδ T cell expansion and αβ T cell depletion. The in-vivo T-cell depletion method depletes (in-vivo) the alloreactive T cells that would otherwise increase the risk of GvHD.
Claims34
A method for allogenic hematopoietic stem cell transplantation (HSCT) comprising the steps of:
- (i) administering to a subject on day 0 a minimally manipulated haploidentical hematopoietic stem cell graft infusion comprising peripheral blood stem cells (PBSC);
- (ii) administering to the subject an agent which provides in vivo T cell depletion;
- (iii) optionally expanding a population of γδ T cells ex vivo;
- and
- (iv) administering to the subject a second graft infusion comprising T cells enriched in γδ T cells and depleted in αβ T cells.
A method for allogenic hematopoietic stem cell transplantation (HSCT) comprising the steps of:
- (i) administering to a subject on day 0 a haploidentical hematopoietic stem cell graft infusion comprising peripheral blood stem cells (PBSC);
- (ii) administering to the subject an agent which provides in vivo T cell depletion;
- (iii) optionally expanding a population of γδ T cells ex vivo;
- and
- (iv) administering to the subject a second graft infusion comprising T cells enriched in γδ T cells and depleted in β T cells.
Dependencies are inferred from the claim text.