Shrimp feed and methods of use thereof
AssigneeOrganicin Scientific, Inc.
InventorsGriffin O'Driscoll, Mathew Mitchell, Margaret Riley
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to methods and compositions for preventing or treating a disease or one or more symptoms thereof caused by pathogenic Vibrio in a shrimp in need thereof. Compositions of the disclosure include a bacteriocin (e.g., Nisin) and shrimp feed.
Claims76
A composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin;
- and
- shrimp feed,
- wherein the composition prevents or treats a disease or one or more symptoms thereof caused by pathogenic Vibrio in a shrimp in need thereof.
The composition of any one of the preceding claims, wherein the shrimp feed comprises:
- anchovy meal, soybean meal, fish oil, tuna oil, wheat flour, krill meal, wheat gluten meal, choline chloride with choline, cholesterol, soy lecithin, Ca(H2PO4)2, salt, Rovimix-stay-C 35, shrimp pre-mix, methionine, threonine, lysine, carboxymethylcellulose, and calcium propionate.
The composition of any one of the preceding claims, wherein the bacteriocin comprises from about 0.0001% to 20% wt/v of the composition.
The composition of any one of the preceding claims, wherein the bacteriocin comprises from about 1×10−3 mg/g to 200 mg/g of the composition.
The composition of any one of the preceding claims, wherein the Nisin comprises from about 0.0001% to 10% wt/v of the composition.
The composition of any one of the preceding claims, wherein the:
- a. shrimp feed comprises about 99.99% wt/v of the composition;
- and
- b. the bacteriocin comprises about 0.01% w/v of the composition.
The composition of any one of the preceding claims, wherein the Nisin comprises Nisin A, Nisin E, Nisin F, Nisin G, Nisin H, Nisin J, Nisin O, Nisin P, Nisin Q, Nisin U, Nisin U2, Nisin P, Nisin Z, combinations thereof, and engineered variants thereof.
The composition of claim 113, wherein the Nisin comprises Nisin Z.
A composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin Z, and wherein the bacteriocin comprises about 0.01% w/v of the composition;
- and
- shrimp feed, wherein the shrimp feed comprises about 99.99% wt/v of the composition, and wherein:
- a. anchovy meal comprises about 26.5% wt/v of the shrimp feed;
- b. soybean meal comprises about 30.4% wt/v of the shrimp feed;
- c. fish oil and tuna oil comprise about 1.8% wt/v of the shrimp feed;
- d. wheat flour comprises about 27.27% N wt/v of the shrimp feed;
- e. krill meal comprises about 2% wt/v of the shrimp feed;
- f. wheat gluten meal comprises about 3.5% wt/v of the shrimp feed;
- g. choline chloride with choline comprises about 1% wt/v of the shrimp feed;
- h. cholesterol comprises about 0.03% wt/v of the shrimp feed;
- i. soy lecithin comprises about 1.5% wt/v of the shrimp feed;
- j. Ca(H2PO4)2 comprises about 2% wt/v of the shrimp feed;
- k. salt comprises about 0.6% wt/v of the shrimp feed;
- l. Rovimix-stay-C 35 comprises about 0.1% wt/v of the shrimp feed;
- m. shrimp pre-mix comprises about 0.4% wt/v of the shrimp feed;
- n. methionine comprises about 0.2% wt/v of the shrimp feed;
- o. threonine comprises about 0.3% v wt/v of the shrimp feed;
- p. lysine comprises about 0.3% wt/v of the shrimp feed;
- q. carboxymethylcellulose comprises about 2% wt/v of the shrimp feed;
- and
- r. calcium propionate comprises about 0.1% wt/v of the shrimp feed,
- wherein the composition prevents or treats a disease or one or more symptoms thereof caused by pathogenic Vibrio in a shrimp in need thereof.
A composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin Z, and wherein the bacteriocin comprises about 0.01% w/v of the composition;
- EDTA, wherein the EDTA comprises about 0.01% wt/v of the composition;
- and
- shrimp feed, wherein the shrimp feed comprises about 99.98% wt/v of the composition, and wherein:
- a. anchovy meal comprises about 26.5% wt/v of the shrimp feed;
- b. soybean meal comprises about 30.4% wt/v of the shrimp feed;
- c. fish oil and tuna oil comprise about 1.8% wt/v of the shrimp feed;
- d. wheat flour comprises about 27.27% wt/v of the shrimp feed;
- e. krill meal comprises about 2% wt/v of the shrimp feed;
- f. wheat gluten meal comprises about 3.5% wt/v of the shrimp feed;
- g. choline chloride with choline comprises about 1% wt/v of the shrimp feed;
- h. cholesterol comprises about 0.03% wt/v of the shrimp feed;
- i. soy lecithin comprises about 1.5% wt/v of the shrimp feed;
- j. Ca(H2PO4)2 comprises about 2% wt/v of the shrimp feed;
- k. salt comprises about 0.6% wt/v of the shrimp feed;
- l. Rovimix-stay-C 35 comprises about 0.1% wt/v of the shrimp feed;
- m. shrimp pre-mix comprises about 0.4% wt/v of the shrimp feed;
- n. methionine comprises about 0.2% wt/v of the shrimp feed;
- o. threonine comprises about 0.3% wt/v of the shrimp feed;
- p. lysine comprises about 0.3% wt/v of the shrimp feed;
- q. carboxymethylcellulose comprises about 2% wt/v of the shrimp feed;
- and
- r. calcium propionate comprises about 0.1% wt/v of the shrimp feed,
- wherein the composition prevents or treats a disease or one or more symptoms thereof caused by pathogenic Vibrio in a shrimp in need thereof.
The composition of any one of the preceding claims, wherein the bacteriocin further comprises:
- a Class I, Class II, and/or Class III bacteriocin.
The composition of any one of the preceding claims, wherein the bacteriocin and shrimp feed are combined.
The composition of any one of the preceding claims, wherein the composition is administered to the shrimp in need thereof.
The composition of any one of the preceding claims, wherein the pathogenic Vibrio comprise Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio vulnificus, Vibrio cholera, Vibrio harveyi, Vibrio fischeri, Vibrio anguillarum, Vibrio spledidus, Vibrio nerves, Vibrio tubiashii, Vibrio campbellii, Vibrio aestuarianus, Vibrio pelagius, Vibrio mediterranei, Vibrio diazotrophicus, Vibrio damselae, Vibrio mimicus, and Vibrio cholera.
The composition of any one of the preceding claims, wherein the disease comprises:
- acute hepatopancreatic necrosis disease (AHPND), vibriosis, loose shell syndrome (LSS), tail necrosis, shell disease, red disease, white gut disease (WGD), white tail disease, white feces disease (WFD), luminous bacterial disease, and necrotizing hepatopancreatitis.
The composition of any one of the preceding claims, wherein the composition does not comprises an antibiotic.
The composition of any one of the preceding claims, wherein the shrimp in need thereof is a farmed shrimp.
A method of preventing or treating a disease or one or more symptoms thereof caused by pathogenic Vibrio in a shrimp in need thereof, the method comprising
- administering a composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin;
- and
- shrimp feed.
A method of preventing or treating a disease or one or more symptoms thereof caused by pathogenic Vibrio in a shrimp in need thereof, the method comprising
- administering a composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin;
- shrimp feed;
- and
- EDTA.
A method of reducing a concentration of pathogenic Vibrio in a shrimp in need thereof, the method comprising administering a composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin;
- and
- shrimp feed.
A method of reducing a concentration of pathogenic Vibrio in a shrimp in need thereof, the method comprising administering a composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin;
- shrimp feed;
- and
- EDTA.
The method of any one of claims 39-46, wherein the shrimp feed comprises:
- anchovy meal, soybean meal, fish oil, tuna oil, wheat flour, krill meal, wheat gluten meal, choline chloride with choline, cholesterol, soy lecithin, Ca(H2PO4)2, salt, Rovimix-stay-C 35, shrimp pre-mix, methionine, threonine, lysine, carboxymethylcellulose, and calcium propionate.
A method of reducing a concentration of pathogenic Vibrio in a shrimp in need thereof, the method comprising administering a composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin Z, and wherein the bacteriocin comprises about 0.01% w/v of the composition;
- and
- shrimp feed, wherein the shrimp feed comprises about 99.99% wt/v of the composition, and wherein:
- a. anchovy meal comprises about 26.5% N wt/v of the shrimp feed;
- b. soybean meal comprises about 30.4% wt/v of the shrimp feed;
- c. fish oil and tuna oil comprise about 1.8% wt/v of the shrimp feed;
- d. wheat flour comprises about 27.27% wt/v of the shrimp feed;
- e. krill meal comprises about 2% wt/v of the shrimp feed;
- f. wheat gluten meal comprises about 3.5% wt/v of the shrimp feed;
- g. choline chloride with choline comprises about 1% wt/v of the shrimp feed;
- h. cholesterol comprises about 0.03% wt/v of the shrimp feed;
- i. soy lecithin comprises about 1.5% wt/v of the shrimp feed;
- j. Ca(H2PO4)2 comprises about 2% wt/v of the shrimp feed;
- k. salt comprises about 0.6% wt/v of the shrimp feed;
- l. Rovimix-stay-C 35 comprises about 0.1% wt/v of the shrimp feed;
- m. shrimp pre-mix comprises about 0.4% wt/v of the shrimp feed;
- n. methionine comprises about 0.2% wt/v of the shrimp feed;
- o. threonine comprises about 0.3% wt/v of the shrimp feed;
- p. lysine comprises about 0.3% wt/v of the shrimp feed;
- q. carboxymethylcellulose comprises about 2% wt/v of the shrimp feed;
- and
- r. calcium propionate comprises about 0.1% wt/v of the shrimp feed.
A method of preventing or treating a disease or one or more symptoms thereof caused by pathogenic Vibrio in a shrimp in need thereof, the method comprising
- administering a composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin Z, and wherein the bacteriocin comprises about 0.01% w/v of the composition;
- and
- shrimp feed, wherein the shrimp feed comprises about 99.99% wt/v of the composition, and wherein:
- a. anchovy meal comprises about 26.5% wt/v of the shrimp feed;
- b. soybean meal comprises about 30.4% wt/v of the shrimp feed;
- c. fish oil and tuna oil comprise about 1.8% wt/v of the shrimp feed;
- d. wheat flour comprises about 27.27% wt/v of the shrimp feed;
- e. krill meal comprises about 2% wt/v of the shrimp feed;
- f. wheat gluten meal comprises about 3.5% wt/v of the shrimp feed;
- g. choline chloride with choline comprises about 1% wt/v of the shrimp feed;
- h. cholesterol comprises about 0.03% wt/v of the shrimp feed;
- i. soy lecithin comprises about 1.5% wt/v of the shrimp feed;
- j. Ca(H2PO4)2 comprises about 2% wt/v of the shrimp feed;
- k. salt comprises about 0.6% wt/v of the shrimp feed;
- l. Rovimix-stay-C 35 comprises about 0.1% wt/v of the shrimp feed;
- m. shrimp pre-mix comprises about 0.4% wt/v of the shrimp feed;
- n. methionine comprises about 0.2% wt/v of the shrimp feed;
- o. threonine comprises about 0.3% wt/v of the shrimp feed;
- p. lysine comprises about 0.3% wt/v of the shrimp feed;
- q. carboxymethylcellulose comprises about 2% wt/v of the shrimp feed;
- and
- r. calcium propionate comprises about 0.1% wt/v of the shrimp feed.
A method of reducing a concentration of pathogenic Vibrio in a shrimp in need thereof, the method comprising administering a composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin Z, and wherein the bacteriocin comprises about 0.01% w/v of the composition;
- EDTA, wherein the EDTA comprises about 0.01% wt/v of the composition;
- and
- shrimp feed, wherein the shrimp feed comprises about 99.98% wt/v of the composition, and wherein:
- a. anchovy meal comprises about 26.5% wt/v of the shrimp feed;
- b. soybean meal comprises about 30.4% wt/v of the shrimp feed;
- c. fish oil and tuna oil comprise about 1.8% wt/v of the shrimp feed;
- d. wheat flour comprises about 27.27% wt/v of the shrimp feed;
- e. krill meal comprises about 2% wt/v of the shrimp feed;
- f. wheat gluten meal comprises about 3.5% wt/v of the shrimp feed;
- g. choline chloride with choline comprises about 1% wt/v of the shrimp feed;
- h. cholesterol comprises about 0.03% wt/v of the shrimp feed;
- i. soy lecithin comprises about 1.5% wt/v of the shrimp feed;
- j. Ca(H2PO4)2 comprises about 2% wt/v of the shrimp feed;
- k. salt comprises about 0.6% wt/v of the shrimp feed;
- l. Rovimix-stay-C 35 comprises about 0.1% wt/v of the shrimp feed;
- m. shrimp pre-mix comprises about 0.4% wt/v of the shrimp feed;
- n. methionine comprises about 0.2% wt/v of the shrimp feed;
- o. threonine comprises about 0.3% wt/v of the shrimp feed;
- p. lysine comprises about 0.3% wt/v of the shrimp feed;
- q. carboxymethylcellulose comprises about 2% wt/v of the shrimp feed;
- and
- r. calcium propionate comprises about 0.1% wt/v of the shrimp feed.
A method of preventing or treating a disease or one or more symptoms thereof caused by pathogenic Vibrio in a shrimp in need thereof, the method comprising
- administering a composition comprising:
- a bacteriocin, wherein the bacteriocin comprises Nisin Z, and wherein the bacteriocin comprises about 0.01% w/v of the composition;
- EDTA, wherein the EDTA comprises about 0.01% wt/v of the composition;
- and
- shrimp feed, wherein the shrimp feed comprises about 99.98% wt/v of the composition, and wherein:
- a. anchovy meal comprises about 26.5% wt/v of the shrimp feed;
- b. soybean meal comprises about 30.4% wt/v of the shrimp feed;
- c. fish oil and tuna oil comprise about 1.8% wt/v of the shrimp feed;
- d. wheat flour comprises about 27.27% N wt/v of the shrimp feed;
- e. krill meal comprises about 2% wt/v of the shrimp feed;
- f. wheat gluten meal comprises about 3.5% wt/v of the shrimp feed;
- g. choline chloride with choline comprises about 1% wt/v of the shrimp feed;
- h. cholesterol comprises about 0.03% wt/v of the shrimp feed;
- i. soy lecithin comprises about 1.5% wt/v of the shrimp feed;
- j. Ca(H2PO4)2 comprises about 2% wt/v of the shrimp feed;
- k. salt comprises about 0.6% wt/v of the shrimp feed;
- l. Rovimix-stay-C 35 comprises about 0.1% wt/v of the shrimp feed;
- m. shrimp pre-mix comprises about 0.4% wt/v of the shrimp feed;
- n. methionine comprises about 0.2% wt/v of the shrimp feed;
- o. threonine comprises about 0.3% N wt/v of the shrimp feed;
- p. lysine comprises about 0.3% wt/v of the shrimp feed;
- q. carboxymethylcellulose comprises about 2% wt/v of the shrimp feed;
- and
- r. calcium propionate comprises about 0.1% wt/v of the shrimp feed.
Dependencies are inferred from the claim text.