Low fiber pennycress meal, seeds, and methods of making
AssigneesCovercress Inc., The Board of Trustees of Illinois State University, Regents of the University of Minnesota
InventorsTim Ulmasov, Gary Hartnell, John C. Sedbrook, Michael David Marks, Ratan Chopra, Maliheh Esfahanian
Life of this patent
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Abstract
Pennycress seed, seed lots, and seed meal having reduced fiber content and improved suitability for use in producing animal feed are provided.
Claims80
A composition comprising non-defatted pennycress seed meal comprising an acid detergent fiber (ADF) content of 5% to 20% by dry weight.
A composition comprising defatted pennycress seed meal comprising an acid detergent fiber (ADF) content of 7% to 25% by dry weight.
Pennycress seed meal comprising an acid detergent fiber (ADF) content of 5% to 20% by dry weight, wherein the seed meal is non-defatted.
Pennycress seed meal comprising an acid detergent fiber (ADF) content of 7% to 25% by dry weight, wherein the seed meal is defatted.
Pennycress seed cake comprising an acid detergent fiber (ADF) content of 7% to 25% by dry weight.
A seed lot comprising a population of pennycress seeds that comprise an acid detergent fiber (ADF) content of 5% to 20% by dry weight.
A method of making a pennycress seed lot comprising the steps of:
- (a) introducing at least one loss-of-function mutation in at least one endogenous wild-type pennycress gene encoding a polypeptide selected from the group consisting of SEQ ID NO:2, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76, 79, 172, and allelic variants thereof;
- (b) selecting germplasm that is homozygous for said loss-of-function mutation;
- and,
- (c) harvesting seed from the homozygous germplasm, thereby obtaining a seed lot, wherein said seed lot comprises an acid detergent fiber (ADF) content of 5% to 20% by dry weight.
A method of making a pennycress seed lot comprising the steps of:
- (a) introducing at least one transgene that suppresses expression of at least one endogenous wild-type pennycress gene encoding a polypeptide selected from the group consisting of SEQ ID NO:
- 2, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76, 79, 172, and allelic variants thereof into a pennycress plant genome;
- (b) selecting a transgenic plant line that comprises said transgene;
- and,
- (c) harvesting seed from the transgenic plant line, thereby obtaining a seed lot, wherein said seed lot comprises an acid detergent fiber (ADF) content of 5% to 20% by dry weight.
Dependencies are inferred from the claim text.