Methods and compositions for tomato brown rugose fruit virus resistance in tomato
OwnerJohannes Hendrikus Maria Barten, Mélissa Christine Cantet +4inventors
InventorsJohannes Hendrikus Maria Barten, Mélissa Christine Cantet, Maria Leontine Hop, Benjamin C. Hunter, Maria Belen Salleres Neira, Antoon Stekelenburg
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Abstract
Tomato (Solanum lycopersicum) plants exhibiting resistance to resistance-breaking isolates of Tomato Brown Rugose Fruit Virus (ToBRFV) are provided, together with methods of producing, identifying, or selecting plants or germplasm with a ToBRFV resistance phenotype. Such plants include tomato plants comprising introgressed genomic regions conferring pest resistance.
Claims35
A Solanum lycopersicum plant comprising:
- (a) a Tm1 resistance gene on chromosome 2;
- (b) a recombinant chromosomal segment on chromosome 11, wherein said chromosomal segment comprises a Tomato Brown Rugose Fruit Virus (ToBRFV) resistance allele;
- and
- (c) a ToBRFV resistance gene on chromosome 8 encoding a ToBRFV resistance protein comprising a polypeptide sequence of SEQ ID NO:18 or 19;
- wherein the plant exhibits increased resistance to ToBRFV, including to resistance-breaking ToBRFV isolates.
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-11. (canceled)
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A method of producing a tomato plant exhibiting increased resistance to resistance-breaking Tomato Brown Rugose Fruit Virus (ToBRFV) isolates, comprising introgressing into a plant a Tm1 resistance gene on chromosome 2, a recombinant chromosomal segment on chromosome 11, wherein said chromosomal segment comprises a ToBRFV resistance allele, or a ToBRFV resistance gene on chromosome 8 encoding a ToBRFV resistance protein comprising a polypeptide sequence of SEQ ID NO:18 or 19, wherein the tomato plant exhibits increased resistance to resistance-breaking ToBRFV isolates.
-23. (canceled)
A method for controlling or preventing ToBRFV symptoms during crop production, wherein the ToBRFV symptoms are caused by resistance-breaking Tomato Brown Rugose Fruit Virus (ToBRFV) isolates, comprising:
- a) obtaining a population of Solanum lycopersicum plants comprising a ToBRFV resistance gene on chromosome 8 encoding a ToBRFV resistance protein comprising a polypeptide sequence of SEQ ID NO:19;
- b) growing the Solanum lycopersicum plants;
- and
- c) harvesting fruit of the Solanum lycopersicum plants.
A method for improving tomato crop yield in an area comprising resistance-breaking Tomato Brown Rugose Fruit Virus (ToBRFV) isolates, the method comprising:
- a) growing a population of Solanum lycopersicum plants comprising a ToBRFV resistance gene on chromosome 8 encoding a ToBRFV resistance protein comprising a polypeptide sequence of SEQ ID NO:19;
- and
- b) harvesting fruit of the Solanum lycopersicum plants, wherein the tomato crop yield of said population of Solanum lycopersicum plants is increased as compared to the yield of a population of Solanum lycopersicum plants lacking the ToBRFV resistance gene.
-32. (canceled)
A recombinant DNA segment from Solanum lycopersicum comprising a Tomato Brown Rugose Fruit Virus (ToBRFV) resistance allele from Solanum habrochaites that confers increased resistance to ToBRFV, wherein said recombinant DNA segment lacks an allele genetically linked thereto that confers an orange mature fruit color phenotype when present in a plant.
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A method of producing a tomato plant with improved Tomato Brown Rugose Fruit Virus (ToBRFV) resistance, comprising introgressing into said plant at least one ToBRFV resistance allele within a recombinant chromosomal segment flanked in the genome of said plant by marker locus M12 (SEQ ID NO:12) and marker locus M14 (SEQ ID NO:14) on chromosome 8, wherein said introgressed ToBRFV resistance allele confers to said plant increased resistance to ToBRFV compared to a plant not comprising said allele, and wherein said recombinant chromosomal segment lacks an allele genetically linked thereto that confers an orange mature fruit color phenotype when present, and wherein said introgressing comprises marker-assisted selection.
-41. (canceled)
Dependencies are inferred from the claim text.