System and method for imaging of tissue samples
OwnerTyler RISOM, Mike REICHELTinventors
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Abstract
The present disclosure involves generating a view of a tissue section that combines a scanning electron microscope image with a mass spectrometry image of the tissue section. Specifically, a scanning electron microscope image of at least a first portion of an ultramicrotome tissue section of a tissue sample is generated using a scanning electron microscope. Also, a mass spectrometry image of at least a second portion of the ultramicrotome tissue section is generated using a mass spectrometry imaging platform, where the mass spectrometry image depicts an antibody labeling of at least one element. The scanning electron microscope image is registered with the mass spectrometry image.
Claims20
A method, comprising:
- generating a scanning electron microscope image of at least a first portion of an ultramicrotome tissue section of a tissue sample using a scanning electron microscope;
- generating a mass spectrometry image of at least a second portion of the ultramicrotome tissue section using a mass spectrometry imaging platform, where the mass spectrometry image depicts an antibody labeling of at least one element;
- and
- registering the scanning electron microscope image with the mass spectrometry image.
A system, comprising:
- a scanning electron microscope image generated using a scanning electron microscope and stored in at least one memory, the scanning electron microscope image depicting at least a first portion of an ultramicrotome tissue section of a tissue sample;
- a mass spectrometry image generated using a mass spectrometry imaging platform and stored in the at least one memory, the mass spectrometry image depicting at least a second portion of the ultramicrotome tissue section;
- and
- at least one processor circuit configured to execute instructions, the instructions causing the at least one processor circuit to register the scanning electron microscope image with the mass spectrometry image.
A non-transitory, computer-readable medium comprising machine-readable instructions that, when executed by a processor of a computing device, cause the computing device to:
- register a scanning electron microscope image with a mass spectrometry image to generate a multilayer image, where the scanning electron microscope image depicts at least a first portion of an ultramicrotome tissue section of a tissue sample using a scanning electron microscope, and the mass spectrometry image depicts at least a second portion of the ultramicrotome tissue section using a mass spectrometry imaging platform, the second portion of the ultramicrotome tissue section at least partially overlapping the first portion of the ultramicrotome tissue section.
Dependencies are inferred from the claim text.