Method and device for microscopy

Microscopy apparatus, comprising:

• a light field microscope having a light source for emitting excitation light,
• an illumination beam path for guiding the excitation light onto or into a sample,
• a two-dimensionally spatially resolving detector for detecting light emitted by the sample,
• a detection beam path at least having a microscope objective and a multi-lens array for imaging light emitted by the sample onto the detector and
• a control and evaluation unit for controlling the light source and the detector and for evaluating the measurement data obtained by the detector, wherein the control and evaluation unit is configured
• A) to control the light source and the detector to record one image of the sample, the image comprising a set of simultaneously recorded partial images;
• B) to establish at least one aberration of the imaging system of the light field microscope from only such partial images simultaneously recorded in step A),
• C) to perform one or both of steps D) and E) below using the at least one aberration of the imaging system established in step B):
• D) reconstruct a three-dimensional image of the sample from the set of partial images with at least partial correction of the at least one aberration established in step B);
• and
• E) establish improved settings of adjustable components of the imaging system which influence wavefronts of the propagated light, on the basis of the at least one aberration established in step B).