Confocal microscopy apparatus

A confocal microscopy apparatus, comprising:

• a generation source of structured light;
• a disk group comprising at least a first rotating disk and a second rotating disk, configured to receive said structured light beam and to transmit a resulting excitation beam to an optics of a microscope focused on a plane of a sample, said disk group being optically interposed between said source and the plane of the sample;
• wherein said first rotating disk and second rotating disk respectively comprise:
• a disk-shaped substrate composed of an optically transparent material and comprising a first planar surface and a second planar surface, respectively opposite, and a mask with pattern arranged on one of the first planar surface and the second planar surface, said mask comprising at least one sector provided with one or more holes, wherein the mask with pattern or an external surface thereof is composed of a highly black material opaque to light;
• an acquisition sensor configured to detect a fluorescent beam emitted from said plane of the sample;
• and
• movement means configured to move said first rotating disk and second rotating disk in such a way that the resulting excitation beam is created by the passage of said structured light beam through at least one opening resulting from the overlapping of said holes, wherein each opening is resulting from the overlapping of a respective first hole included in the first rotating disk and of a respective second hole included in the second rotating disk,
• wherein said movement means is configured to move said first rotating disk and second rotating disk according to a mutual translation motion,
• wherein the area of said openings is variable by adjusting the reciprocal position of said first rotating disk and second rotating disk,
• wherein said movement means comprises:
• a motor configured to be integrally coupled with said first rotating disk, and
• motion transmission means configured to kinematically couple said motor with said second rotating disk,
• wherein said motion transmission means is configured to perform a translation of said second rotating disk with respect to said first rotating disk,
• wherein said motion transmission means is configured to perform a linear sliding displacement of the second rotating disk with respect to the first rotating disk, wherein the rotation axes of each first and second rotating disk remain coincident, through the use of prismatic guides and an axial conical pusher.