Spatial Proteomics Analysis Using Wavelength-Specific Click Chemistry for Subcellular Resolution Localization

A spatial proteomics device comprising:

• (a) a matrix of independently controllable laser sources, each laser source configured to emit light at a distinct wavelength selected from UV (250-400 nm), blue (400-500 nm), green (500-600 nm), and red (600-750 nm);
• (b) a positioning system configured to direct each laser beam to predetermined spatial coordinates on a biological sample with accuracy within 1 micrometer;
• (c) a sample stage configured to hold biological samples including cell cultures, tissue sections, and FFPE slides;
• (d) a detection system configured to record the spatial coordinates and wavelength of each laser activation event;
• (e) a control system configured to coordinate laser activation timing and spatial positioning for simultaneous multi-wavelength photoactivation.

A method for clinical spatial proteomics analysis of formalin-fixed paraffin-embedded (FFPE) tissue samples, comprising:

• (a) applying photoactivatable click chemistry reagents uniformly across an FFPE tissue slide;
• (b) directing a matrix of wavelength-specific laser beams to predetermined regions of clinical interest on said slide;
• (c) activating said reagents in a location-specific manner based on the wavelength applied to each region;
• (d) performing in situ click chemistry reactions to label proteins within activated tissue regions;
• (e) overlaying spatial imaging data with proteomic analysis results;
• (f) correlating protein localization data with histopathological features for clinical diagnosis.