Applications of hepatocyte co-culture system

A kit for plating hepatocytes, comprising

• (a) a first cryovial comprising primary hepatocytes, wherein the primary hepatocytes are neonatal or juvenile hepatocytes;
• (b) a second cryovial comprising endothelial cells;
• (c) a third cryovial comprising stromal cells;
• and
• (d) an instruction for preparing plated hepatocytes with the primary hepatocytes, the endothelial cells and the stromal cells according to a method comprising:
• (i) applying the primary hepatocytes to a surface in the presence of feeder cells, wherein the feeder cells are the endothelial cells and the stromal cells,
• (ii) co-culturing the applied hepatocytes with the feeder cells after step (i), and
• (iii) forming one or more hepatocyte clusters by the co-cultured hepatocytes on the feeder cells, wherein the feeder cells are attached to the surface, wherein at least 85% of the co-cultured hepatocytes are in the one or more hepatocyte clusters.

A kit for plating hepatocytes, comprising

• (a) a first cryovial comprising primary hepatocytes;
• (b) a second cryovial comprising endothelial cells;
• (c) a third cryovial comprising stromal cells;
• (d) a fourth cryovial comprising Kupffer cells;
• and
• (e) an instruction for preparing plated hepatocytes with the primary hepatocytes, the endothelial cells and the stromal cells according to a method comprising:
• (i) applying the primary hepatocytes to a surface in the presence of feeder cells, wherein the feeder cells are the endothelial cells and the stromal cells,
• (ii) co-culturing the applied hepatocytes with the feeder cells after step (i), and
• (iii) forming one or more hepatocyte clusters by the co-cultured hepatocytes on the feeder cells, wherein the feeder cells are attached to the surface, wherein at least 85% of the co-cultured hepatocytes are in the one or more hepatocyte clusters.