Inline enrichment and separation of biomolecules in microfluidic devices

A microfluidic device, comprising:

• a channel, configured to accommodate a mixed analyte sample mixed with a gel solution, the channel having a first end and a second end;
• a first reservoir coupled to the first end of the channel, the first reservoir being configured to accommodate a first reservoir solution;
• a second reservoir coupled to the second of the channel, the second reservoir being configured to accommodate a second reservoir solution;
• a first electrode arranged in the first reservoir;
• and
• a second electrode arranged in the second reservoir;
• wherein the first electrode and the second electrode are configured to apply an electric field across the microfluidic device.

A computer-readable medium storing computer-readable instructions executable by one or more processors, that when executed by the one or more processors, causes the one or more processors to perform acts comprising:

• loading a mixed analyte sample mixed with a gel solution into a channel of a microfluidic device,
• the channel having a first end and a second end,
• the microfluidic device having a first reservoir coupled to the first end of the channel and a second reservoir coupled to the second end of the channel, and
• the microfluidic device having a first electrode and a second electrode;
• providing a first reservoir solution in the first reservoir;
• providing a second reservoir solution in the second reservoir;
• and
• applying an electric field across the microfluidic device.