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US 2026/0287548 A1Published application
Capillary-array-electrophoresis device
AssigneeHitachi High-Tech Corporation
InventorsTakashi ANAZAWA, Ryoji INABA, Shuhei YAMAMOTO
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Abstract
When laser-irradiation portions of N capillaries with capillary numbers n=1, 2, . . . , and N are arranged on a same plane, a laser-irradiation intensity of each capillary is denoted by L(n), and an output intensity of each capillary by a computer when a light emitting substance having an equal concentration exists inside each capillary is denoted by H(n), an absolute value of an average value of a second derivative of H(n) becomes smaller than an absolute value of an average value of a second derivative of L(n) for any refractive index n3 of a separation medium in a range of 1.33≤n3≤1.41 by digital correction by the computer, which changes according to the refractive index n3 (see FIG. 11).
Claims14
A capillary array electrophoresis device comprising:
- a laser light source configured to emit a laser beam;
- a capillary array configured such that laser irradiation portions of N capillaries collectively irradiated with the laser beam are substantially arranged on a same array plane, where N is an integer of 2 or more;
- an optical system configured to collectively measure fluorescence emitted from the N capillaries;
- and
- a computer configured to perform predetermined processing on fluorescence intensities of the N capillaries measured by the optical system and to output the processed fluorescence intensities,
- wherein an outer radius of the N capillaries in the laser irradiation portion is defined as R, an inner radius of the N capillaries is defined as r, a refractive index of an external medium is defined as n1, a refractive index of a material is defined as n2, and a refractive index of an internal medium is defined as n3,
- wherein capillary numbers n=1, 2, . . . , and N are assigned to the respective N capillaries in the laser irradiation portion in an arrangement order from one end of the arrangement, and
- wherein, with respect to (a) a degree of downward convexity of an emission fluorescence intensity distribution I(n), that is an emission fluorescence intensity as a function of the capillary number n when a fluorescent substance of equal concentration exists inside the N capillaries in the laser irradiation portion or (b) a degree of downward convexity of a measured fluorescence intensity distribution M(n), that is a measured fluorescence intensity as a function of the capillary number n when a fluorescent substance of equal concentration exists inside the N capillaries in the laser irradiation portion, a degree of downward convexity of an output fluorescence intensity distribution H(n), that is an output fluorescence intensity as a function of the capillary number n output by the computer when a fluorescent substance of equal concentration exists inside the N capillaries in the laser irradiation portion is small.
5 more elements
A capillary array electrophoresis device comprising:
- a laser light source configured to emit a laser beam;
- a capillary array configured such that laser irradiation portions of N capillaries collectively irradiated with the laser beam are substantially arranged on a same array plane, where N is an integer of 2 or more;
- an optical system configured to collectively measure fluorescence emitted from the N capillaries;
- and
- a computer configured to perform predetermined processing on fluorescence intensities of the N capillaries measured by the optical system and to output the processed fluorescence intensities,
- wherein an outer radius of the N capillaries in the laser irradiation portion is defined as R, an inner radius of the N capillaries is defined as r, a refractive index of an external medium is defined as n1, a refractive index of a material is defined as n2, and a refractive index of an internal medium is defined as n3,
- wherein capillary numbers n=1, 2, . . . , and N are assigned to the respective N capillaries in the laser irradiation portion in an arrangement order from one end of the arrangement,
- wherein a second-order coefficient when an emission fluorescence intensity distribution (n), that is an emission fluorescence intensity as a function of the capillary number n,
- or a second-order coefficient when a measured fluorescence intensity distribution M(n) represented as a function of n of an emission fluorescence intensity of the capillary number n,
- is approximated by quadratic function is defined as A when a fluorescent substance of equal concentration exists inside the N capillaries in the laser irradiation portion,
- a second-order coefficient when an output fluorescence intensity distribution H(n), that is an output fluorescence intensity as a function of the capillary number n output by the computer, is approximated by quadratic function is defined as B when a fluorescent substance of equal concentration exists inside the N capillaries in the laser irradiation portion, and
- the computer is configured to perform a processing to satisfy |A|>|B|.
9 more elements
Dependencies are inferred from the claim text.