Electrochemical nucleic acid detection sensing element and electrochemical nucleic acid detection method based on rpa and crispr/cas

Abstract

The present application relates to the field of electrochemical analysis and detection, and in particular to an electrochemical nucleic acid detection sensing element and electrochemical nucleic acid detection method based on RPA and CRISPR/Cas. According to the present application, RPA technology, a CRISPR/Cas tool, and electrochemical detection technology are combined, and additionally, an electrochemical active molecule and a modification group are linked to a reporter molecule in a CRISPR/Cas reaction system, such that construction of an immobilization-free and homogeneous electrochemical nucleic acid detection method is realized, and detection of the target nucleic acid can be realized.

Dependencies are inferred from the claim text.