Electrochemical aptamer biosensor for detecting phenylalanine and/or tyrosine
AssigneeSeoul Women's University Industry-University Cooperation Foundation
InventorsChang Seuk LEE, JAEMIN JEONG, INSEON YU
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Abstract
The present disclosure relates to an electrochemical aptamer biosensor capable of rapidly and easily detecting phenylalanine and/or tyrosine present in a sample. It is possible to rapidly and easily detect the presence of phenylalanine and/or tyrosine in a sample by using the electrochemical aptamer biosensor of the present disclosure to effectively monitor information on tyrosine accumulation in the body of patients with phenylketonuria, thereby contributing to the treatment and management of patients with phenylketonuria.
Claims13
An electrochemical aptamer biosensor for detecting phenylalanine or tyrosine, comprising:
- a reduced graphene oxide substrate;
- a phenylalanine-specific binding aptamer immobilized to the substrate;
- and
- a complementary polynucleotide including a sequence partially complementary to the aptamer and tagged with an electrochemical labeling material at a terminal.
A method for manufacturing an electrochemical aptamer biosensor for detecting phenylalanine or tyrosine, comprising:
- step 1, immobilizing a phenylalanine-specific binding aptamer on the surface of a reduced graphene oxide electrode;
- and
- step 2, hybridizing the immobilized aptamer with a complementary polynucleotide comprising a sequence partially complementary to the aptamer and tagged with an electrochemical labeling material at a terminal thereof.
A method for manufacturing an electrochemical aptamer biosensor for detecting phenylalanine or tyrosine, comprising:
- step 1, hybridizing a phenylalanine-specific binding aptamer with a complementary polynucleotide comprising a sequence partially complementary to the aptamer and tagged with an electrochemical labeling material at a terminal thereof;
- and
- step 2, coating the hybrid material of step 1 above on the surface of a reduced graphene oxide electrode.
Dependencies are inferred from the claim text.