Luminescence signal amplification
AssigneeUniversity of Wyoming
InventorDebashis DUTTA
Life of this patent
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Abstract
Embodiments described herein generally relate to luminescent detection devices and systems, and to methods for luminescent detection. In an embodiment is provided a device for luminescence detection. The device includes a first layer comprising an optically-transparent material, the first layer having a first surface and a second surface opposite the first surface. The device further includes a second layer comprising a well, the well comprising: an opening, the opening formed in the second layer and facing the first surface of the first layer; a bottom wall disposed opposite the opening; and a sidewall extending between the opening and the bottom wall. The device further includes a metal layer disposed over at least a portion of the bottom wall and the sidewall of the well.
Claims20
A device for luminescence detection, comprising:
- a first layer comprising an optically-transparent material, the first layer having a first surface and a second surface opposite the first surface;
- a second layer comprising:
- a well comprising:
- an opening, the opening formed in the second layer and facing the first surface of the first layer;
- a bottom wall disposed opposite the opening;
- and
- a sidewall extending between the opening and the bottom wall, the sidewall having a first region extending from the opening and a second region extending from the first region to the bottom wall, the first region having a substantially constant or constant radius and the second region having a first radius adjacent to the first region and a second radius adjacent to the bottom wall, wherein the second radius is smaller than the first radius;
- and
- an optically reflective metal layer disposed over at least a portion of the bottom wall and the sidewall of the well.
A luminescence detection system, comprising:
- a device comprising:
- a first layer comprising an optically-transparent material, the first layer having a first surface and a second surface opposite the first surface;
- a second layer comprising:
- a well comprising:
- an opening, the opening formed in the second layer and facing the first surface of the first layer;
- a bottom wall disposed opposite the opening;
- and
- a sidewall extending between the opening and the bottom wall, the sidewall having a first region extending from the opening and a second region extending from the first region to the bottom wall, the first region having a substantially constant or constant radius and the second region having a first radius adjacent to the first region and a second radius adjacent to the bottom wall, wherein the second radius is smaller than the first radius;
- and
- an optically reflective metal layer disposed over at least a portion of the bottom wall and the sidewall of the well;
- and
- a detector adapted to be optically coupled to the device.
A method of detecting an analyte in a sample, the method comprising:
- introducing a sample to a well of a device under conditions effective to detect an analyte present in the sample, the device further comprising:
- a first layer comprising an optically-transparent material, the first layer having a first surface and a second surface opposite the first surface;
- a second layer comprising:
- the well comprising:
- an opening, the opening formed in the second layer and facing the first surface of the first layer;
- a bottom wall disposed opposite the opening;
- and
- a sidewall extending between the opening and the bottom wall, the sidewall having a first region extending from the opening and a second region extending from the first region to the bottom wall, the first region having a substantially constant or constant radius and the second region having a first radius adjacent to the first region and a second radius adjacent to the bottom wall, wherein the second radius is smaller than the first radius;
- and
- an optically reflective metal layer disposed over at least a portion of the bottom wall and the sidewall of the well;
- and
- detecting the presence or absence of the analyte in the well with a detector.
Dependencies are inferred from the claim text.