Goniometric flow cytometry

A goniometric flow cytometry system, comprising:

• a sample flow path configured for flowing one or more samples through a sample plane;
• an input light source configured to illuminate the sample plane;
• and
• a set of one or more optics configured to (i) direct forward scatter (FSC) light to one or more image sensors and cause presentation of an FSC angular scattering field from the FSC light at the one or more image sensors and (ii) direct side scatter (SSC) light to the one or more image sensors and cause presentation of an SSC angular scattering field from the SSC light at the one or more image sensors.

A method for performing goniometric flow cytometry (GFC), comprising:

• flowing a sample through a sample plane of a flow cytometry system;
• for the sample:
• capturing a forward scatter (FSC) angular scattering field from an FSC signal from the sample plane using one or more image sensors;
• capturing a side scatter (SSC) angular scattering field from an SSC signal from the sample plane using the one or more image sensors;
• determining an FSC angular scattering signal based on the FSC angular scattering field captured using the one or more image sensors;
• determining an SSC angular scattering signal based on the SSC angular scattering field captured using the one or more image sensors;
• constructing a 2D scattering metric for the sample by combining the FSC angular scattering signal with the SSC angular scattering signal;
• and
• generating a GFC score for the sample based on the 2D scattering metric for the sample and one or more 2D scattering metrics for one or more other samples;
• and
• assigning one or more classifications to the sample based on the GFC score for the sample.