Methods for epitype enriched pcr screening

8-17. (canceled)

(canceled)

25-27. (canceled)

32-85. (canceled)

A composition comprising:

• a nucleic acid template derived from bisulfite converted cell-free DNA obtained from a biological sample of a subject, wherein prior to conversion, the cell-free DNA comprises a sequence between 50-500 nucleotides, between 75-400 nucleotides, between 100-300 nucleotides, between 125-250 nucleotides, or between 150-200 nucleotides of a CpG island (CGI), wherein between 0 and X cytosines of the sequence of the cell-free DNA are converted to a uracil in the nucleic acid template, wherein X represents a total number of CpG sites in the sequence of the cell-free DNA;
• and
• a nucleic acid epitype probe hybridized to the nucleic acid template, wherein the nucleic acid epitype probe comprises a sequence complementary to the sequence of the nucleic acid template.

A composition comprising a nucleic acid epitype probe comprising:

• a nucleic acid sequence complementary to a sequence of a nucleic acid template derived from bisulfite converted cell-free DNA obtained from a biological sample of a subject, wherein prior to conversion, the cell-free DNA comprises a sequence between 50-500 nucleotides, between 75-400 nucleotides, between 100-300 nucleotides, between 125-250 nucleotides, or between 150-200 nucleotides of a CpG island (CGI), and
• a binding moiety that binds to a solid support, a detection moiety, and/or a barcode.

89-103. (canceled)