Internal standard nucleic acids for genomic or metagenomic analysis
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsYuji SEKIGUCHI, Dieter TOURLOUSSE, Akiko OHASHI
Life of this patent
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Abstract
Provided is a nucleic acid molecule comprising an artificial nucleic acid sequence and/or a complementary sequence thereof or a partial fragment sequence thereof the artificial nucleic acid sequence consisting of: (1) one copy each of artificial genes encoding the following non-naturally occurring sequences (a) to (p); (2) artificial intergenic sequences for linking the artificial genes, each independently consisting of a non-naturally occurring random sequence of 10 to 60 nucleotides in length; and (3) a front spacer sequence and an end spacer sequence, each independently consisting of a non-naturally occurring random sequence of 200 to 400 nucleotides in length.
Claims6
A nucleic acid molecule comprising an artificial nucleic acid sequence and/or a complementary sequence thereof, or a partial fragment sequence thereof, the artificial nucleic acid sequence consisting of:
- (1) one copy each of artificial genes encoding the following non-naturally occurring sequences (a) to (p):
- (a) an amino acid sequence of SEQ ID NO:
- 1 and a stop codon;
- (b) an amino acid sequence of SEQ ID NO:
- 2 and a stop codon;
- (c) an amino acid sequence of SEQ ID NO:
- 3 and a stop codon;
- (d) an amino acid sequence of SEQ ID NO:
- 4 and a stop codon;
- (e) an amino acid sequence of SEQ ID NO:
- 5 and a stop codon;
- (f) an amino acid sequence of SEQ ID NO:
- 6 and a stop codon;
- (g) an amino acid sequence of SEQ ID NO:
- 7 and a stop codon;
- (h) an amino acid sequence of SEQ ID NO:
- 8 and a stop codon;
- (i) an amino acid sequence of SEQ ID NO:
- 9 and a stop codon;
- (j) an amino acid sequence of SEQ ID NO:
- 10 and a stop codon;
- (k) an amino acid sequence of SEQ ID NO:
- 11 and a stop codon;
- (l) an amino acid sequence of SEQ ID NO:
- 12 and a stop codon;
- (m) an amino acid sequence of SEQ ID NO:
- 13 and a stop codon;
- (n) an amino acid sequence of SEQ ID NO:
- 14 and a stop codon;
- (o) an amino acid sequence of SEQ ID NO:
- 15 and a stop codon;
- and
- (p) an amino acid sequence of SEQ ID NO:
- 16 and a stop codon:
- (2) artificial intergenic sequences for linking the artificial genes, each independently consisting of a non-naturally occurring random sequence of 10 to 60 nucleotides in length;
- and
- (3) a front spacer sequence and an end spacer sequence, each independently consisting of a non-naturally occurring random sequence of 200 to 400 nucleotides in length.
A nucleic acid molecule comprising an artificial nucleic acid sequence of SEQ ID NO:
- 23 and/or the complementary sequence thereof, or the partial fragment sequence thereof.
Dependencies are inferred from the claim text.