High-yield fructosylated chondroitin-producing Escherichia coli and its application thereof

Abstract

The present disclosure discloses a high-yield fructosylated chondroitin-producing Escherichia coli and its application thereof, belonging to the technical field of microbiology. On the basis of E. coli K4, the present disclosure constructs a strain with enhanced fructosylated chondroitin synthesis capacity by mutating genes encoding key enzymes in a fructosylated chondroitin synthesis pathway. The yield of fructosylated chondroitin produced by this strain reaches 10 g/L in a 5 L fermenter and 15-20 g/L in a 100 L fermenter. The obtained final product has a uniform structure and stable quality, which effectively reduces the production costs and the risk of environmental pollution, thus being suitable for large-scale industrial production applications.

Dependencies are inferred from the claim text.