Method of preparing organosilicon compounds with selective cytochrome p450 variants and related compounds and compositions

Abstract

A method of preparing an organosilicon compound having at least one silicon-bonded carbinol group and/or at least one silanol group with a cytochrome P450 variant that facilitates the oxidization of a silicon-bonded hydrocarbyl group in the presence of an oxidant. The method includes combining the cytochrome P450 variant, an initial organosilicon compound having at least one silicon-bonded hydrocarbyl group, and a cofactor to give a reaction mixture, and exposing the reaction mixture to an oxidant to oxidize the silicon-bonded hydrocarbyl group of the initial organosilicon compound, thereby preparing the organosilicon compound having at least one silicon-bonded carbinol group and/or at least one silanol group. Cytochrome P450 variants suitable for use in the method are also disclosed, along with methods for engineering and optimizing the same. Nucleic acids encoding the cytochrome P450 variants and compositions, expression vectors, and host cells including the same are also disclosed.

Dependencies are inferred from the claim text.