Fusion proteins for improved gene editing
AssigneesNew York Genome Center, Inc., New York University
InventorsNeville E. Sanjana, Wells Burell, Akash Sookdeo
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Abstract
Compositions and methods for improved gene editing are provided. The compositions in fusion proteins comprising a Cas enzyme and at least one domain from a second protein that is ADH4, COMMD4, AEBP2, KLHL20, LMNA, FOXO3, CEP63, RFC3, EVL, ERCC8_isoform1, ERCC8_isoform2, ZNF296, RAD21, KAT5, APOBEC3F_isoform1, APOBEC3F_isoform2, PARN, UBE2B, VHL, RASSF1_isoform1, RASSF1_isoform2, CRX, RAD51C_isoform1, RAD51C_isoform2, RNF14, LMO1, TBX10, ANXA2R, DPF1, BARD1, SWSAP1, XRCC6, DUSP7, or ERCC6L.
Claims30
A fusion protein comprising a Cas enzyme and at least one domain from a second protein that is ADH4, COMMD4, AEBP2, KLHL20, LMNA, FOXO3, CEP63, RFC3, EVL, ERCC8_isoform1, ERCC8 isoform2, ZNF296, RAD21, KAT5, APOBEC3F_isoform1, APOBEC3F_isoform2, PARN, UBE2B, VHL, RASSF1_isoform1, RASSF1_isoform2, CRX, RAD51C_isoform1, RAD51C_isoform2, RNF14, LMO1, TBX10, ANXA2R, DPF1, BARD1, SWSAP1, XRCC6, DUSP7, or ERCC6L, or a domain sharing at least 90%, at least 95%, or at least 99% identity with any one of the second proteins.
The fusion protein of 12, wherein the linker comprises:
- i)
- SGGSSGSGSETPGTSESATPESSGGSSSGGGSGGSGS;
- ii)
- SGGGSGGSGS;
- iii)
- GGGS;
- iv)
- SGSETPGTSESATPES;
- and/or
- v)
- SGGSSGGSSGSETPGTSESATPESSGGSSGGSS.
A fusion protein comprising an endonuclease and at least one domain from a second protein that is ADH4, COMMD4, AEBP2, KLHL20, LMNA, FOXO3, CEP63, RFC3, EVL, ERCC8_isoform1, ERCC8_isoform2, ZNF296, RAD21, KAT5, APOBEC3F_isoform1, APOBEC3F_isoform2, PARN, UBE2B, VHL, RASSF1_isoform1, RASSF1_isoform2, CRX, RAD51C_isoform1, RAD51C_isoform2, RNF14, LMO1, TBX10, ANXA2R, DPF1, BARD1, SWSAP1, XRCC6, DUSP7, or ERCC6L, or a domain sharing at least 90%, at least 95%, or at least 99% identity with any one of the second proteins.
An expression cassette comprising the polynucleotide of any one of claims 15 to 27.
A plasmid comprising the polynucleotide of any one of claim 15 to 27 or the expression cassette according to claim 22.
A composition comprising a lipid nanoparticle (LNP) and the polynucleotide of any one of claims 16 to 27.
Dependencies are inferred from the claim text.