Pseudotyped retroviral vector particle with anti-cd3 display
AssigneeMiltenyi Biotec GmbH
InventorsNora Winter, Nicole Cordes-Paulitz, Thomas Schaser
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Abstract
The present invention provides a pseudotyped retroviral vector particle for activating and transducing T cells, wherein said retroviral vector particle comprises a modulating protein comprising a functional ectodomain comprising an antigen binding domain specific for the antigen CD3, wherein said antigen binding domain specific for the antigen CD3 comprises a humanized and optimized scFV sequence, wherein said retroviral vector particle comprises at least one nucleic acid sequence encoding a transgene, and wherein said retroviral vector particle is a lentiviral or gammaretroviral vector particle. A pharmaceutical composition thereof is also disclosed.
Claims13
1) A pseudotyped retroviral vector particle for activating and transducing T cells, wherein said retroviral vector particle comprises a modulating protein comprising a functional ectodomain comprising an antigen binding domain specific for the antigen CD3, wherein said antigen binding domain specific for the antigen CD3 comprises SEQ ID NO:1 and SEQ ID NO:2, wherein said modulating protein comprises:
- I) said functional ectodomain
- II) a transmembrane domain, wherein said transmembrane domain is the transmembrane domain of CD8, CD28 or PDGFR,
- III) a cytoplasmic domain,
- wherein said retroviral vector particle comprises at least one nucleic acid sequence encoding a transgene, and wherein said retroviral vector particle is a lentiviral or gammaretroviral vector particle.
5) The pseudotyped retroviral vector particle of claim 6, wherein in said retroviral vector particle of B) said protein H of a virus of the Paramyxoviridae family lacks at least one part of the cytoplasmic region of said protein H, and wherein said envelope protein with fusion activity lacks at least one part of the cytoplasmic region of said envelope protein;
- or
- wherein in said retroviral vector particle of C) said protein G of a virus of the Paramyxoviridae family lacks at least one part of the cytoplasmic region of said protein G, and wherein said envelope protein with fusion activity lacks at least one part of the cytoplasmic region of said envelope protein.
8) A composition comprising
- i) a pseudotyped retroviral vector particle for activating and transducing T cells comprising
- a) an envelope protein with antigen-binding activity, wherein said envelope protein is a recombinant protein that does not interact with at least one of its native receptors and is fused at its ectodomain to a polypeptide that specifically binds to a tag of a tagged polypeptide,
- b) an envelope protein with fusion activity,
- c) a modulating protein comprising a functional ectodomain comprising an antigen binding domain specific for the antigen CD3, wherein said antigen binding domain specific for the antigen CD3 comprises SEQ ID NO:1 and SEQ ID NO:2, wherein said modulating protein comprises:
- I) said functional ectodomain
- II) a transmembrane domain, wherein said transmembrane domain is the transmembrane domain of CD8, CD28 or PDGFR,
- III) a cytoplasmic domain,
- ii) said tagged polypeptide, wherein said tagged polypeptide binds specifically to a target antigen expressed on the surface of a T cell, wherein said target antigen is not CD3, and
- wherein said retroviral vector particle comprises at least a nucleic acid sequence encoding a transgene, and wherein said retroviral vector particle is a lentiviral or gammaretroviral vector particle.
Dependencies are inferred from the claim text.