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US 2026/0286391 A1Published application
Vector production
AssigneesOspedale San Raffaele S.R.L., Fondazione Telethon Ets
InventorsAlessio Cantore, Michela Milani, Chiara Simoni, Cesare Canepari
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Abstract
An enveloped viral particle producer or packaging cell, wherein the cell is modified to decrease expression of low density lipoprotein receptor (LDLR) on the surface of the cell.
Claims20
An enveloped viral particle producer or packaging cell, wherein the cell is modified to decrease expression of low density lipoprotein receptor (LDLR) on the surface of the cell.
A method of producing enveloped viral particles comprising the steps:
- (a) introducing a transfer vector and optionally one or more helper vector into a cell;
- (b) introducing a vector comprising a nucleotide sequence encoding pro-protein convertase subtilisin/Kexin type 9 (PCSK9) into the cell;
- (c) culturing the cell under conditions suitable for the production of the enveloped viral particles.
A method of producing enveloped virus-like particles (VLPs) comprising the steps:
- (a) introducing one or more helper vector into a cell;
- (b) introducing a vector comprising a nucleotide sequence encoding pro-protein convertase subtilisin/Kexin type 9 (PCSK9) into the cell;
- and
- (b) culturing the cell under conditions suitable for the production of the enveloped VLPs.
1 more element
Dependencies are inferred from the claim text.