Gene edited maize with altered characteristics
AssigneesVib Vzw, Universiteit Gent, Inari Agriculture Technology, Inc., Instituut Voor Landbouw- En Visserijonderzoek (Ilvo)
InventorsTom Van Hautegem, Hannes Bart Claeys, Frédéric Van Ex, Michael Lee Nuccio, Hilde Nelissen, Dirk Inzé, Tom Ruttink
Life of this patent
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Abstract
The disclosure relates to maize plants with altered characteristics including tolerance to abiotic stress such as drought and/or increased water use efficiency, along with methods of using and making the same.
Claims47
A maize plant comprising a loss-of-function allele of the endogenous maize gene of SEQ ID NO:
- 1, 3, 5, 7, 9, and/or 11, or an allelic variant thereof.
A biological sample comprising a nucleic acid containing a loss-of-function allele of the endogenous maize gene of SEQ ID NO:
- 1, 3, 5, 7, 9, and/or 11, or an allelic variant thereof.
A guide RNA molecule comprising a spacer RNA molecule that targets the endogenous maize gene of SEQ ID NO:
- 1, 3, 5, 7, 9, 11, or an allelic variant thereof.
A method for determining whether a maize plant cell, plant part, plant, or biological sample obtained therefrom comprises a loss-of-function allele of the endogenous maize gene of SEQ ID NO:
- 1, 3, 5, 7, 9, and/or 11, or an allelic variant thereof, the method comprising:
- (i) analyzing a polynucleotide comprising a portion of SEQ ID NO:
- 1, 3, 5, 7, 9, and/or 11, or an allelic variant thereof, or analyzing an RNA encoded by a portion of SEQ ID NO:
- 1, 3, 5, 7, 9, and/or 11, or an allelic variant thereof, from the maize plant cell, plant part, plant, or biological sample, wherein an insertion, deletion, and/or substitution of one or more nucleotides in the polynucleotide or RNA is indicative of the presence of the loss-of-function allele in the maize plant cell, plant part, plant, or biological sample;
- and/or
- (ii) analyzing a polypeptide encoded by SEQ ID NO:
- 1, 3, 5, 7, 9, and/or 11, a portion thereof, or an allelic variant thereof from the maize plant cell, plant part, or plant, wherein an insertion, deletion, and/or substitution of one or more amino acid residues of the polypeptide or a change in the biologic or biochemical activity of the polypeptide is indicative of the presence of the loss-of-function allele in the maize plant cell, plant part, plant, or biological sample.
Dependencies are inferred from the claim text.