Engineering increased suberin levels in plants
AssigneeThe Salk Institute for Biological Studies
InventorsJulie Ann Law, Mina Rostamza, Joseph P. Noel, Suzanne Thomas, Jeffrey Todd Mindrebo, Luke Alden Sarre
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Abstract
The present disclosure provides compositions and methods for increasing suberin production in plants, for example by increasing MYB41 expression in a plant. In some examples, MYB41 expression is by a heterologous promoter, such as a FACT gene promoter, a HORST gene promoter, a GPAT5 gene promoter, a RALPH gene promoter, a ASFT gene promoter, or a MYB84 gene promoter.
Claims34
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a MYB41 amino acid sequence with at least 80% sequence identity to any one of SEQ ID NOs:
- 37-91 and/or a nucleic acid sequence having at least 80% sequence identity to any one of SEQ ID NOs:
- 92-201, operably linked to a nucleic acid sequence encoding a heterologous promoter, wherein expression of the isolated nucleic acid molecule in a plant results in increased levels of suberin as compared to wild-type check plants lacking the isolated nucleic acid molecule.
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-28. (canceled)
-41. (canceled)
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A genetically engineered plant, comprising an isolated nucleic acid molecule encoding a MYB41 amino acid sequence that is selected from the group consisting of SEQ ID NOs:
- 38, 40, 44, 46, 49, 52, 70, 76, 77, 78, and 86, operably linked to a nucleic acid sequence encoding a heterologous promoter, wherein expression of the isolated nucleic acid molecule in a plant results in increased levels of suberin as compared to wild-type check plants lacking the isolated nucleic acid molecule.
A genetically engineered plant, comprising an isolated nucleic acid molecule that is selected from the group consisting of SEQ ID NOs:
- 93, 95, 99, 101, 104, 107, 125, 131, 132, 133, 141 148, 150, 154, 156, 159, 162, 180, 186, 187, 188, and 196, operably linked to a nucleic acid sequence encoding a heterologous promoter, wherein expression of the isolated nucleic acid molecule in a plant results in increased levels of suberin as compared to wild-type check plants lacking the isolated nucleic acid molecule.
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Dependencies are inferred from the claim text.