Modifications of transgenic corn event zm_bcs216090 and methods thereof
OwnerLinda Rymarquisinventor
Life of this patent
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Abstract
A modified corn event ZM_BCS216090 and plants, plant cells, seeds, plant parts, progeny plants and commodity products comprising a modified corn event ZM_BCS216090 are provided. Polynucleotides and sequences specific for a modified corn event ZM_BCS216090, methods for making a modified corn event ZM_BCS216090, methods for making and using plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising a modified corn event ZM_BCS216090, and methods for detecting a modified corn event ZM_BCS216090, or a polynucleotide or DNA sequence specific for a modified corn event ZM_BCS216090, in a DNA molecule or sample, are provided.
Claims63
A recombinant DNA molecule comprising:
- (a) a first nucleotide sequence (i) comprising an expression cassette that encodes a microRNA (miRNA) that suppresses the expression of the endogenous GA20ox3 and GA20ox5 genes in corn, or (ii) selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, and SEQ ID NO:
- 9;
- and
- (b) a second nucleotide sequence that
- (i) comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:
- 17 or 19,
- (ii) is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:
- 17 or 19,
- (iii) is selected from the group consisting of SEQ ID NOs:
- 30-49 and SEQ ID NOs:
- 50-129,
- (iv) comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:
- 18 or 20,
- (v) is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:
- 18 or 20, or
- (vi) is selected from the group consisting of SEQ ID NOs:
- 130-149 and SEQ ID NOS:
- 150-229.
(canceled)
6-13. (canceled)
A recombinant DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe that hybridizes specifically under stringent hybridization conditions with a polynucleotide having a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, and SEQ ID NO:
- 20.
A pair of DNA molecules that can function as DNA primers when used together in an amplification reaction comprising a first DNA molecule and a second DNA molecule, wherein the first DNA molecule and the second DNA molecule are different,
- (a) wherein the first DNA molecule is:
- (i) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 9, or a complement thereof, or
- (ii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:
- 17 or 19, or a complement thereof;
- and
- (b) wherein the second DNA molecule is:
- (i) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 9, or a complement thereof, or
- (ii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:
- 17 or 19, or a complement thereof, or
- (iii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:
- 18 or 20, or a complement thereof.
(canceled)
A method of detecting the presence of a DNA segment diagnostic for a modified corn event ZM_BCS216090 DNA in a sample, said method comprising performing a sequencing reaction with the sample, wherein the production in the sequencing reaction of a target nucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 9, or a complement thereof, is diagnostic for the modified corn event ZM_BCS216090 DNA in the sample.
27-33. (canceled)
A method of producing a progeny corn plant comprising a modified corn event ZM_BCS216090 comprising:
- a) sexually crossing a first modified corn plant that comprises a modified corn event ZM_BCS216090 with itself or a second corn plant;
- b) collecting one or more seeds produced from said cross;
- c) growing said seed to produce one or more progeny plants;
- and
- d) selecting at least a first progeny plant or seed comprising a modified corn event ZM_BCS216090.
46-47. (canceled)
A method of producing a commodity product, said method comprising:
- a) obtaining a modified corn plant, corn plant part, or corn seed comprising a modified corn event ZM_BCS216090;
- and
- b) producing a commodity product from the transgenic corn plant, corn plant part, or corn seed.
A corn plant, corn plant part, or corn seed comprising a DNA molecule or segment functional as a template when tested in a DNA amplification method to produce an amplicon diagnostic for the presence of a modified corn event ZM_BCS216090 DNA.
(canceled)
54-55. (canceled)
(canceled)
A population of transgenic corn plants, wherein each transgenic corn plant comprises a modified corn event ZM_BCS216090.
A method of modifying a corn plant, the method comprising:
- (a) introducing a site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a site-specific nuclease into at least one cell of an explant of a corn plant comprising corn event ZM_BCS216090, or a plant part thereof, to produce a modified corn event ZM_BCS216090 via a targeted genome editing technique;
- and
- (b) developing or regenerating a modified corn plant from the explant, wherein the modified corn plant comprises the modified corn event ZM_BCS216090.
64-74. (canceled)
A method of introducing a target site into a corn plant, the method comprising:
- I:
- (a) introducing a cognate target site into the corn event ZM_BCS216090 locus of at least one cell of a corn plant or corn plant part comprising the corn event ZM_BCS216090 or an explant thereof via a targeted genome editing technique, wherein the cognate target site is identical or similar to an originator target site for a site-specific nuclease present in the corn event ZM_BCS216090 locus, and
- (b) developing or regenerating a modified corn plant comprising a modified corn event ZM_BCS216090 comprising the cognate target site;
- or
- II:
- (a) introducing a cognate target site into the corn event ZM_BCS216090 locus of at least one cell of a corn plant or corn plant part comprising the corn event ZM_BCS216090 or an explant thereof via a targeted genome editing technique to produce a modified corn event ZM_BCS216090 comprising the cognate target site, wherein the cognate target site is identical or similar to an originator target site for a site-specific nuclease present in the corn event ZM_BCS216090 locus, and
- (b) introducing a site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a site-specific nuclease into at least one cell of an explant of a modified corn plant comprising the modified corn event ZM_BCS216090, or a plant part thereof, to produce a further modified corn event ZM_BCS216090 via a targeted genome editing technique, wherein the target site of the site-specific nuclease includes the cognate target site and the originator target site;
- and
- (c) developing or regenerating a second modified corn plant comprising the further modified corn event ZM_BCS216090.
(canceled)
A method of modifying an explant of a corn plant or plant part, the method comprising:
- introducing a site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a site-specific nuclease into at least one cell of an explant of a corn plant or plant part comprising corn event ZM_BCS216090 to produce a modified corn event ZM_BCS216090 into the at least one cell of the explant.
Dependencies are inferred from the claim text.