Method for controlling intracellular localization of sgrna and regulating gene expression

Abstract

A light-controlled element NES-Ms2-Lans5 includes, sequentially in an order from 5′ to 3′, a NES gene fragment, an Ms2 gene fragment, and a Lans5 gene fragment. Each gene fragment is linked via a linker. A light-controlled CRISPR component including the aforementioned light-controlled element, a gene targeting element, and a gene regulatory element is provided. According to the light-controlled CRISPR component, a method for light-controlled target gene expression is provided. A strategy to achieve gene editing by regulating an intracellular localization of sgRNA is firstly provided, enabling activation and expression of specific genes.

Dependencies are inferred from the claim text.