Preparation and compositions of cot-1 nucleic acid
AssigneeIllumina, Inc.
InventorsJian-Sen Li, Xin Sheng, Angelica Marie Barr Schalembier, Stephen Gross
Life of this patent
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Abstract
Presented herein are Cot-1 methods and compositions that may be used for reducing non-specific binding, such as enhancing specific enrichment of target sequences in a nucleic acid library. In an embodiment, a Cot-1 composition may be a modified Cot-1 composition that has sequences with similarity to coding sequences removed. In an embodiment, a Cot-1 composition may be a synthetic Cot-1 composition. In an embodiment, a Cot-1 composition may be a minimal or reduced Cot-1 composition.
Claims14
A method of generating Cot-1 DNA, comprising:
- contacting genomic DNA fragments with a hybridization probe panel under conditions that permit hybridization of probes of the hybridization probe panel to the genomic DNA fragments, wherein the probes of hybridization probe panel are configured to hybridize to coding regions of the genomic DNA fragments;
- separating a first portion of the genomic DNA fragments from a second portion of the genomic DNA fragments based on differential binding to the hybridization probe panel, wherein the first portion is not bound to the probes and the second portion is bound to the probes;
- and
- providing the first portion of the genomic DNA fragments as Cot-1 DNA.
A Cot DNA composition prepared by a method comprising:
- contacting genomic DNA fragments with a hybridization probe panel under conditions that permit hybridization of probes of the hybridization probe panel to the genomic DNA fragments, wherein the probes of hybridization probe panel are configured to hybridize to coding regions of the genomic DNA fragments;
- separating a first portion of the genomic DNA fragments from a second portion of the genomic DNA fragments based on differential binding to the hybridization probe panel, wherein the first portion is not bound to the probes and the second portion is bound to the probes;
- and
- providing the first portion of the genomic DNA fragments as the Cot DNA composition.
Dependencies are inferred from the claim text.