Compositions comprising modified human galactocerebrosidase
AssigneeM6P Therapeutics, Inc.
InventorsLin LIU, Andrew Charles HEDMAN
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Abstract
Provided are modified forms of human Galactocerebrosidase (GALC) enzyme. Included are descriptions of the methods for synthesis of DNA constructs for GALC for the expression of the GALC enzyme with modified amino acid sequence. Additionally, descriptions of GALC enzyme assays are provided that demonstrate higher specific GALC activity for proteins with the modified GALC amino acid sequence.
Claims15
A composition comprising whole or truncated human wild type GALC comprising at least one of the following amino acid substitutions:
- T145 to 1145, T177 to R177, S200 to P200, I208 to E208, S232 to P232, K261 to W261, Y300 to N300, and C322 to S322.
A composition comprising whole or truncated human wild type GALC of amino acid SEQ ID NO. 1 comprising one or more of the following amino acid substitutions:
- T177 to R177, S200 to P200, 1208 to E208, S232 to P232, K261 to W261, Y300 to N300, and C322 to S322.
Dependencies are inferred from the claim text.