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AssigneeEndura S.p.A.
InventorsRoberto VERGA, Simone STASI, Silvia RAPACIOLI, Carla MARCHIORO, Silvia CARLONI, Leni MORONI, Valerio BORZATTA
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The present invention concerns the field of enzyme mutants, and in particular Arthrobacter globiformis esterase mutants suitable for asymmetrical hydrolysis of the cyclopropane derivatives, specifically chrysanthemate ester in any combination of the four stereoisomers in order to selectively obtain (1R,3R)-chrysanthemic acid or a salt thereof. The invention further describes an expression vector including the Arthrobacter globiformis esterase mutant and transformed host microorganisms harboring the vectors. The present invention further relates to the use of Arthrobacter globiformis esterase mutants to selectively obtain (1R,3R)-chrysanthemic acid. Under a further aspect, the invention relates to the use of Arthrobacter globiformis esterase mutants for the asymmetrical hydrolysis of cyclopropane racemate derivatives having at least two chiral stereocenters, preferably for the asymmetrical hydrolysis of cyclopropane racemate derivatives having at least two chiral stereocenters.
1-15. (canceled)
An Arthrobacter globiformis esterase mutant having a sequence comprising mutations S315M or S315F of SEQ ID NO:
An expression vector including an Arthrobacter globiformis esterase mutant nucleotide sequence chosen from the group consisting of SEQ ID NO:
Dependencies are inferred from the claim text.