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AssigneeConception Biosciences, Inc.
InventorsTisha E. BOHR, Alyssa J. MILLER, Pablo Ignacio HURTADO GONZALEZ, Karmen Bianka SERES, Erika PAULSON
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Provided in some aspects are methods for generating primordial follicles from human and other mammalian germ cells, with high efficiency, high cellular quality and high reproducibility. In some aspects, provided are methods and culture system for generating ovarian organoids, such as cell aggregates composed of both somatic and germ cells from primary and/or stem cell-derived sources, which are competent to progress primordial germ cells to oocytes that enter meiosis and are competent for follicle assembly. In some aspects, provided embodiments include a step to culture ovarian organoids in a fully defined serum-free media and in conditions with low defined serum protein components overall.
A method of producing primordial follicles, the method comprising:
A method of producing oogonia and/or oocytes, the method comprising:
The method of 25 or 26 wherein the supplement medium is added to the base medium to a final concentration of 7.5% or less.
An ovarian organoid comprising one or more primordial follicles, wherein:
An ovarian organoid, wherein the ovarian organoid is derived from a cell mixture of ovarian germ cells and somatic cells in culture, and the ovarian organoid comprises at least 5, at least 10, at least 50, at least 100, or at least 500 oocytes and/or oogonia cells derived from the ovarian germ cells, wherein the ovarian germ cells are from human or non-human primate.
Dependencies are inferred from the claim text.