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US 2026/0286311 A1Published application
Culture media and conditions for in vitro expansion and/or maturation of hepatocytes
AssigneeSatellite Biosciences, Inc.
InventorsSunil Kumar MALLANNA, Maxwell P. VAN BUSKIRK, Soujanya S. KARANTH
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Abstract
Provided herein are completely defined two-dimensional culture conditions, including culture supplements and requirements for an extracellular matrix, facilitating the expansion of and long-term maintenance of primary human hepatocytes, as well as culture conditions and media for maturation of hepatocytes.
Claims17
A method for culturing primary human hepatocytes, the method comprising the step of culturing one or more hepatocytes in contact with an extracellular matrix (ECM) in the presence of an expansion medium comprising a basal medium for human cells to which is added:
- Wnt3a,
- an R-spondin,
- a fibroblast growth factor (FGF),
- an epidermal growth factor (EGF),
- a hepatocyte growth factor (HGF), and
- a transforming growth factor-alpha (TGF-alpha), wherein the culturing step is performed on a two-dimensional surface, and wherein the two-dimensional surface is coated with the ECM, and wherein the hepatocytes are adherently attached to the surface during the culturing step.
3 more elements
Dependencies are inferred from the claim text.