Compositions and methods for cell culture applications
OwnerAndrea Vernengo, Jennifer Weiserinventors
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Abstract
The present disclosure generally relates methods and compositions comprising thermosensitive hydrogel compositions that can be used for embedded patterning of cells, cell aggregates, or organoids. The present disclosure further generally relates to methods of inducing cell condensation into geometrically directed tissue structures and simultaneously producing therapeutic cell secretomes.
Claims31
A thermosensitive hydrogel composition comprising:
- (a) a support phase, wherein the support phase comprises:
- (i) a primary component comprising a continuous or granular polymeric phase dissolved in an aqueous solution, further wherein the primary component is capable of Bingham plastic rheological behavior in the presence of an aqueous media;
- (ii) a secondary component comprising a biocompatible thermally-desolubilizable polymer or co-polymer that exists in an extended form below a critical solution temperature (CST) that is lower than the normal body temperature of a mammal and in a condensed form at or above the CST;
- (b) an embedded phase, wherein the embedded phase comprises at least one uncrosslinked polymer and further comprises at least one cell, wherein the embedded phase is capable of extrusion into the support phase at or below the CST of the support phase.
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A method for culturing a cell, wherein the method comprises:
- (a) providing a thermosensitive hydrogel composition, wherein the thermosensitive hydrogel composition comprises:
- (i) a support phase comprising:
- (1) a primary component, wherein the primary component comprises a continuous or granular polymeric phase dissolved in an aqueous solution, further wherein the primary component is capable of Bingham plastic rheological behavior in the presence of an aqueous media;
- (2) a secondary component, wherein the secondary component comprises a biocompatible thermally-desolubilizable polymer or co-polymer that exists in an extended form below a critical solution temperature (CST) that is lower than the normal body temperature of the mammal and in a condensed form at or above the CST;
- (b) depositing an embedded phase into the support phase at or below the CST of the biocompatible thermally-desolubilizable polymer or co-polymer of the support phase, wherein the embedded phase comprises at least one uncrosslinked polymer and further comprises a cell;
- (c) culturing the at least one cell, wherein during the culturing, the temperature of the composition is increased to at or above the CST of the biocompatible thermally-desolubilizable polymer or co-polymer of the support phase following deposition of the embedded phase;
- (d) optionally collecting the secretome expressed by the cell.
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Dependencies are inferred from the claim text.