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AssigneeStemcell Technologies Canada Inc.
InventorsOlivia NEEF, Kimberly SNYDER, Arwen HUNTER, Adam HIRST, Matthew WONG
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The present disclosure relates to cell culture applications, and more specifically to cell culture applications for expanding cells, such as stem cells (e.g. PSC). Methods include seeding cells in a first vessel and optionally transferring cells from the first vessel to a second vessel. Cells of the first vessel, and as applicable in the second vessel, are contacted with at least one exogenously added extracellular matrix or extracellular matrix protein. Methods of this disclosure yield significantly and rapidly expanded populations of cells, while maintaining cell quality. Methods of this disclosure may also improve cell plating efficiencies of cells to be significantly and rapidly cultured/expanded in monolayer.
1) A method of expanding cells in monolayer, comprising providing a first suspension of cells;
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16) An expedited method of expanding cells in a monolayer,
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Dependencies are inferred from the claim text.